@article{3174200,
    title = "Plasma biomarkers for the identification of women at risk for
early-onset preeclampsia",
    author = "Kolialexi, Aggeliki and Tsangaris, George Th and Sifakis, Stavros and and Gourgiotis, Dimitris and Katsafadou, Aggeliki and Lykoudi, Alexandra and and Marmarinos, Antonios and Mavreli, Danai and Pergialiotis, Vassilis and and Fexi, Dimitra and Mavrou, Ariadni and Papaioanou, George K. and and Papantoniou, Nikolas",
    journal = "Expert Review of Proteomics",
    year = "2017",
    volume = "14",
    number = "3",
    pages = "269-276",
    publisher = "TAYLOR & FRANCIS LTD LONDON",
    issn = "1478-9450, 1744-8387",
    doi = "10.1080/14789450.2017.1291345",
    keywords = "Preeclampsia; early onset preeclampsia; proteomics; 2-DE; mass
spectrometry; biomarkers",
    abstract = "Background: To identify potential biomarkers in the 1st trimester of
pregnancy for the identification of women destined to develop early
onset preeclampsia (EOPE).Methods: Blood samples were obtained from
pregnant women at 11-13weeks of gestation. Women were followed up until
delivery. Five samples from EOPE complicated pregnancies and 5 from
unaffected ones were analysed using 2-DE and MALDI-TOF-TOF MS/MS. The
altered expression of selected proteins was verified by ELISA in an
extended sample cohort.Results: Twelve proteins were differentially
expressed in the plasma of women who subsequently developed EOPE as
compared to controls. Alpha-1-antitrypsin (A1AT), CD5 antigen-like
molecule (CD5L) Keratin, type I cytoskeletal 9 (K1C9), Myeloid cell
nuclear differentiation antigen (MNDA), Transferrin (TRFE) and Vitamin
D-binding protein (VTDB) were up-regulated with fold changes 3.14, 2.18,
1.53, 1.53, 4.26 3.38 respectively, whereas Alpha-2-HS-glycoprotein
(FETUA), Beta-2-glycoprotein 1 (APOH), Complement factor B (CFAB),
Haptoglobin (HPT), Vitronectin (VTNC) and Zinc-alpha-2-glycoprotein
(ZA2G) were down-regulated with fold changes -0.38, -0.76, -0.24, -0.47,
-0.23, and -0.50 respectively. The down-regulation of APOH, VTNC and HPT
was verified using ELISA.Conclusions: The differentially expressed
proteins represent potential biomarkers for the early screening for
EOPE. Follow-up experiments however are necessary for evaluation."
}