@incollection{3188454,
    title = "In Situ Detection of miRNAs in Senescent Cells in Archival Material",
    author = "Pateras, Ioannis S. and Theodorou, Sofia D. P. and Chouliari, Niki V. and and Veroutis, Dimitris and Passias, Panagiotis-Georgios and Evangelou, and Konstantinos and Gorgoulis, Vassilis G.",
    booktitle = "SENOLYTICS IN DISEASE, AGEING AND LONGEVITY",
    publisher = "SPRINGER INTERNATIONAL PUBLISHING AG",
    year = "2020",
    isbn = "978-3-030-44903-2; 978-3-030-44902-5",
    pages = "147-162",
    doi = "10.1007/978-3-030-44903-2_8",
    keywords = "Senescence; Co-detection; miRNAs; GL13 (SenTraGor (TM));
Senotherapeutics",
    abstract = "The detection of senescent cells has been challenging. We have recently
developed a novel hybrid histo-/immunohisto-chemical method that can
bypass several constraints of detection of senescent cells. It is based
on the development of a novel reagent called GL13 (SenTraGorTM) that
binds lipofuscin, a non-degradable metabolic by-product that is known as
a hallmark of senescence. This chapter provides a unique approach to
detect formalin fixed paraffin embedded tissues miRNAs in senescent
GL13-reactive cells in routine. Given the significant role of miRNAs in
senescent programs, this approach enables for the first time to monitor
miRNAs in the context of senescence in situ in archival material.
Notably, this assay improves our capacity to detect in vivo senescent
cells which favors rationalization of senotherapeutic drugs. Although
these agents are in early clinical trials, their introduction in routine
practice will transform healthcare as we know it, bringing to the fore
the necessity for precise detection of senescent cells in tissues."
}