@article{3220302,
    title = "Voluntary physical activity mitigates alveolar bone loss in mice with ligature-induced experimental periodontitis",
    author = "Bertolini, N.O. and Pereira, G.J.S. and Silva, V.O. and de Molon, R.S. and Morari, J. and Velloso, L.A. and Andrade, E.F. and Pereira, L.J. and Moura, R.F.",
    journal = "ARCHIVES OF ORAL BIOLOGY",
    year = "2022",
    volume = "140",
    publisher = "Elsevier Ireland Ltd",
    issn = "0003-9969",
    doi = "10.1016/j.archoralbio.2022.105451",
    abstract = "Objective: To investigate the effect of voluntary physical activity (VPA) on inflammatory profile and the progression of experimental periodontal disease (PD) in mice. Methods: Male C57BL/6 mice were randomly distributed into Control; VPA; PD and PD/VPA groups. We registered VPA (total volume of revolutions) and average speed (revolutions/minute) in a free running wheel for 30 days. On the 15th day, animals from the PD and PD/VPA groups received ligatures on the upper second molars bilaterally. On the 30th day animals were euthanized, and PD progression was assessed by measuring alveolar bone loss (ABL - the linear distance between the cemento-enamel junction and the alveolar bone crest on the teeth buccal surface). Gene expression of RANKL (kappa nuclear factor B receptor) OPG (osteoprotegerin), IL-1β (interleukin 1 beta), IL-6 (interleukin 6) and TNF-α (tumor necrosis factor alpha) were evaluated by real-time PCR (quantitative Polymerase Chain Reaction - relative gene expression). Results: The total volume of physical activity and the activity speed decreased along the seven days after ligature-placement (p < 0.05), returning to a similar pattern in relation to VPA group. Ligature placement produced significant bone resorption, and increased RANKL, IL-1β, IL-6 and TNF-α expression. VPA reduced ABL (p < 0,05) and the expression of TNF-α and IL-1β, whereas increased OPG expression. Conclusion: Animals induced to PD with access to the VPA wheel presented both lower gingival inflammation and less alveolar bone resorption in comparison to animals without access to the wheel. © 2022 Elsevier Ltd"
}