TY - JOUR
TI - Synthesis, structural characterization and biological studies of novel mixed ligand Ag(I) complexes with tri-phenylphosphine and aspirin or salicylic acid
AU - M. Poyraza, C. Bantia, N. Kourkoumelis, V. Dokoroud, M.J. Manose, M. Simčič, S. Golič Grdadolnik, T. Mavromoustakos, I.I. Verginadis, K. Charalabopoulos, S.K. Hadjikakou
JO - INORGANICA CHIMICA ACTA
PY - 2011
VL - 375
TODO - 1
SP - 114-121
PB - Elsevier
SN - 0020-1693
TODO - 10.1016/j.ica.2011.04.032
TODO - Bioinorganic chemistry Silver(I) complexes Aspirin Crystal structures Cytotoxic activity STD 1 H NMR
TODO - Two new mixed ligand silver(I) complexes of formulae {[Ag(tpp)3(asp)](dmf)} (1) (aspH = o-acetylsalicylic acid and tpp = triphenylphosphine) and [Ag(tpp)2(o-Hbza)] (2) (o-HbzaH = o-hydroxy-benzoic acid)
were synthesized and characterized by elemental analyses, spectroscopic techniques and X-ray crystallography at ambient conditions. Three phosphorus and one carboxylic oxygen atoms from a de-protonated aspirin ligand in complex 1 and two phosphorus and two carboxylic oxygen atoms from a
chelating o-Hbza anion in complex 2 form a tetrahedral geometry around Ag(I) ions in both complexes.
Complexes 1 and 2 and the silver(I) nitrate, tpp, aspNa and o-HbzaH were tested for their in vitro cytotoxic activity against leiomyosarcoma cells (LMS), human breast adenocarcinoma cells (MCF-7) and normal human fetal lung fibroblasts (MRC-5) cells with Thiazolyl Blue Tetrazolium Bromide (MTT) assay. For
both cell lines 1 and 2 were found to be more active than cisplatin. Additionally, 1 and 2 exhibit lower
activity on cell growth proliferation of MRC-5 cells. The type of LMS cell death caused by 1 and 2 were
evaluated in vitro by use of flow cytometry assay. The results show that at concentrations of 1.5 and
1.9 lV of complex 1, 44.1% and 69.4%, respectively of LMS cells undergo programmed cell death (apoptosis). When LMS cells were treated with 1.6 and 2.3 lM of 2, LMS cells death was by 29.6% and 81.3%,
respectively apoptotic. Finally, the influence of the complexes 1 and 2, upon the catalytic peroxidation of
linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX) was kinetically and theoretically studied. The binding of 1 and 2 towards LOX was also investigated by Saturation Transfer Difference
(STD) 1
H NMR experiments
ER -