TY - JOUR
TI - Degradation mechanism of 2,4-dichlorophenol by fungi isolated from marine invertebrates
AU - Nikolaivits, E.
AU - Agrafiotis, A.
AU - Baira, E.
AU - Le Goff, G.
AU - Tsafantakis, N.
AU - Chavanich, S.A.
AU - Benayahu, Y.
AU - Ouazzani, J.
AU - Fokialakis, N.
AU - Topakas, E.
JO - International Journal of Molecular Sciences
PY - 2020
VL - 21
TODO - 9
SP - null
PB - MDPI AG
SN - 1422-0067
TODO - 10.3390/ijms21093317
TODO - 2,4 dichlorophenol;  2,4-dichlorophenol;  catechol 1,2 dioxygenase;  chlorophenol;  fungal protein, Article;  Ascidiacea;  bacterium isolation;  biocatalysis;  biocatalyst;  biodegradation;  biogenesis;  bioremediation;  biotransformation;  dechlorination;  denitrification;  enzyme activity;  enzyme degradation;  fungus isolation;  glycosylation;  high performance liquid chromatography;  human;  Human respiratory syncytial virus;  laparoscopic cholecystectomy;  liquid chromatography-mass spectrometry;  marine invertebrate;  mass fragmentography;  metabolite;  metabolomics;  nonhuman;  Penicillium chrysogenum;  Phanerochaete;  polymerase chain reaction;  soil analysis;  Trypanosomatidae;  ultra performance liquid chromatography;  animal;  aquatic species;  Basidiomycetes;  enzymology;  invertebrate;  isolation and purification;  metabolism;  microbiology;  physiology;  symbiosis;  water pollutant, Animals;  Aquatic Organisms;  Basidiomycota;  Biodegradation, Environmental;  Catechol 1,2-Dioxygenase;  Chlorophenols;  Fungal Proteins;  Humans;  Invertebrates;  Symbiosis;  Water Pollutants, Chemical
TODO - 2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
ER -