TY - JOUR
TI - Synthesis and angiogenetic activity in the chick chorioallantoic membrane model of thymosin beta-15
AU - Koutrafouri, V.
AU - Leondiadis, L.
AU - Ferderigos, N.
AU - Avgoustakis, K.
AU - Livaniou, E.
AU - Evangelatos, G.P.
AU - Ithakissios, D.S.
JO - Peptides
PY - 2003
VL - 24
TODO - 1
SP - 107-115
PB - 
SN - 0196-9781
TODO - 10.1016/S0196-9781(02)00282-6
TODO - angiogenic factor;  phorbol 12 acetate 13 myristate;  resin;  thymosin beta15;  unclassified drug, amino acid analysis;  angiogenesis;  article;  biological model;  breast cancer;  cancer tissue;  chicken;  chorioallantois;  concentration response;  drug synthesis;  human;  in vivo study;  mass spectrometry;  nonhuman;  peptide synthesis;  priority journal;  prostate cancer;  protein localization;  protein stability;  protein structure, Allantoin;  Amino Acid Sequence;  Animals;  Chick Embryo;  Chorion;  Chromatography, High Pressure Liquid;  Molecular Sequence Data;  Neovascularization, Physiologic;  Spectrometry, Mass, Electrospray Ionization;  Thymosin
TODO - Thymosin beta-15 (Tβ15), a 44 amino acid peptide (MW=5173) localized in human prostate and breast cancer tissues was successfully synthesized in multigram quantities using Fmoc solid-phase peptide synthesis. The synthesized product was shown to have the right structure by ESI and MALDI mass spectral techniques and amino acid analysis. Relatively high yield was achieved, which might be due to enhanced acid stability of the p-cyanotrityl resin used. The effect of the synthesized Tβ15 on the angiogenesis process was investigated using the chick chorioallantoic membrane (CAM) in vivo model. At concentrations above 1μg/10μl per disc, Tβ15 exhibited a positive effect on angiogenesis, comparable to the effect of the intense angiogenetic factor phorbol 12-myristate 13-acetate at a standard concentration of 0.1μg/10μl per disc. The results of this study contribute to the further elucidation of the biological regulatory role of thymosin peptides and provide helpful information in the investigation of their possible therapeutic potential. © 2002 Elsevier Science Inc. All rights reserved.
ER -