TY - JOUR
TI - Real-life evaluation of a COVID-19 rapid antigen detection test in hospitalized children
AU - Eleftheriou, I.
AU - Dasoula, F.
AU - Dimopoulou, D.
AU - Lebessi, E.
AU - Serafi, E.
AU - Spyridis, N.
AU - Tsolia, M.
JO - Journal of Medical Virology
PY - 2021
VL - 93
TODO - 10
SP - 6040-6044
PB - John Wiley and Sons Inc
SN - 0146-6615, 1096-9071
TODO - 10.1002/jmv.27149
TODO - coronavirus nucleocapsid protein;  virus antigen, adolescent;  antigen detection;  Article;  child;  cohort analysis;  controlled clinical trial;  controlled study;  coronavirus disease 2019;  diagnostic test accuracy study;  female;  Greece;  hospitalization;  human;  intermethod comparison;  lateral flow immunochromatography;  major clinical study;  male;  nasopharyngeal swab;  prospective study;  reverse transcription polymerase chain reaction;  sensitivity and specificity;  symptom;  diagnosis;  false negative result;  false positive result;  hospitalization;  hospitalized child;  immunoassay;  infant;  isolation and purification;  nasopharynx;  pandemic;  preschool child;  procedures;  virology, Adolescent;  Antigens, Viral;  Child;  Child, Hospitalized;  Child, Preschool;  COVID-19;  COVID-19 Serological Testing;  False Negative Reactions;  False Positive Reactions;  Female;  Hospitalization;  Humans;  Immunoassay;  Infant;  Male;  Nasopharynx;  Pandemics;  Prospective Studies;  SARS-CoV-2;  Sensitivity and Specificity
TODO - Rapid antigen detection (RAD) tests for the detection of SARS-CoV-2 are simpler, faster, and less expensive than the reverse-transcription polymerase chain reaction (RT-PCR) that is currently considered the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19). The objective of this study was to determine the performance of the PANBIO COVID-19 Ag RAD (Abbott) test, a lateral flow immunoassay that detects the nucleocapsid protein, using as a reference RT-PCR method the Cobas®8800 System (Roche Diagnostics). This prospective study was conducted in a tertiary Children's Hospital and included individuals aged ≤16 years with COVID-19-related symptoms or epidemiological criteria for COVID-19. Two nasopharyngeal samples were collected to perform the PANBIO RAD test and RT-PCR. Of 744 children included, 51 (6.86%) had a positive RT-PCR result. The RAD test detected 42 of 51 PCR-positive children while there were no false-positive results. The overall sensitivity and specificity were 82.35% (95% CI, 71.9%–92.8%) and 100%, respectively. Sensitivity was >95% in symptomatic children. The assay performed poorly in asymptomatically infected children. In agreement with previous studies in adults, the PANBIO RAD test can be useful in screening for COVID-19 in children admitted with symptoms suggestive of the disease, especially in the first days of the illness. © 2021 Wiley Periodicals LLC
ER -