TY - JOUR
TI - Detection in two hospitals of transferable ceftazidimeavibactam resistance in Klebsiella pneumoniae due to a novel VEB β-lactamase variant with a Lys234Arg substitution, Greece, 2019
AU - Voulgari, E.
AU - Kotsakis, S.D.
AU - Giannopoulou, P.
AU - Perivolioti, E.
AU - Tzouvelekis, L.S.
AU - Miriagou, V.
JO - Eurosurveillance
PY - 2020
VL - 25
TODO - 2
SP - null
PB - European Centre for Disease Prevention and Control (ECDC)
SN - 1025-496X, 1560-7917
TODO - 10.2807/1560-7917.ES.2020.25.2.1900766
TODO - avibactam plus ceftazidime;  beta lactamase;  carbapenemase;  antiinfective agent;  avibactam, ceftazidime drug combination;  azabicyclo derivative;  bacterial protein;  beta lactamase;  carbapenem derivative;  ceftazidime, antibiotic resistance;  antibiotic sensitivity;  bacterial strain;  bacterium isolate;  bacterium isolation;  Escherichia coli;  female;  Greece;  hospital;  human;  Klebsiella pneumoniae;  molecular cloning;  nonhuman;  phenotype;  plasmid;  Review;  whole genome sequencing;  antibiotic resistance;  bacterial genome;  drug combination;  drug effect;  enzymology;  genetics;  isolation and purification;  Klebsiella infection;  Klebsiella pneumoniae;  metabolism;  microbial sensitivity test;  microbiology;  multidrug resistance;  mutation, Anti-Bacterial Agents;  Azabicyclo Compounds;  Bacterial Proteins;  beta-Lactamases;  Carbapenems;  Ceftazidime;  Drug Combinations;  Drug Resistance, Bacterial;  Drug Resistance, Multiple, Bacterial;  Genome, Bacterial;  Greece;  Humans;  Klebsiella Infections;  Klebsiella pneumoniae;  Microbial Sensitivity Tests;  Mutation;  Plasmids;  Whole Genome Sequencing
TODO - Two ceftazidime-avibactam (CAZ-AVI)-resistant Klebsiella pneumoniae carbapenemase (KPC)-positive K. pneumoniae strains, including one pandrug resistant, were isolated in 2019 from two Greek hospitals. The strains were sequence types (ST)s 258 and 147 and both harboured similar self-transmissible IncA/C2 plasmids encoding a novel Lys234Arg variant of the Vietnamese extended-spectrum β-lactamase (VEB)-1, not inhibited by AVI (VEB-25). Conjugal transfer of VEB-25-encoding plasmids to Escherichia coli yielded CAZ-AVI-resistant clones, supporting that VEB-25 is directly linked to the derived phenotype. © 2020 European Centre for Disease Prevention and Control (ECDC). All rights reserved.
ER -