TY - JOUR
TI - Early Response Roles for Prolactin Cortisol and Circulating and Cellular Levels of Heat Shock Proteins 72 and 90α in Severe Sepsis and SIRS
AU - Vardas, K.
AU - Apostolou, K.
AU - Briassouli, E.
AU - Goukos, D.
AU - Psarra, K.
AU - Botoula, E.
AU - Tsagarakis, S.
AU - Magira, E.
AU - Routsi, C.
AU - Nanas, S.
AU - Briassoulis, G.
JO - BioMed Research International
PY - 2014
VL - 2014
TODO - null
SP - null
PB - Hindawi Publishing Corporation
SN - 2314-6133, 2314-6141
TODO - 10.1155/2014/803561
TODO - corticotropin;  gamma interferon;  heat shock protein 72;  heat shock protein 90;  heat shock protein 90alpha;  hydrocortisone;  interleukin 10;  interleukin 17;  interleukin 6;  lactic acid;  prolactin;  unclassified drug;  corticotropin;  heat shock protein 72;  heat shock protein 90;  HSP90alpha protein, human;  hydrocortisone;  prolactin, acute stress;  adult;  APACHE;  Article;  clinical article;  concentration (parameters);  controlled study;  corticotropin blood level;  enzyme linked immunosorbent assay;  extracellular space;  female;  flow cytometry;  fluorescence analysis;  hospital admission;  human;  hydrocortisone blood level;  immunoassay;  intensive care unit;  male;  monocyte;  prolactin blood level;  protein expression;  sepsis;  Simplified Acute Physiology Score;  stress;  systemic inflammatory response syndrome;  blood;  case control study;  middle aged;  physiological stress;  sepsis;  systemic inflammatory response syndrome, Adrenocorticotropic Hormone;  Adult;  Case-Control Studies;  Female;  HSP72 Heat-Shock Proteins;  HSP90 Heat-Shock Proteins;  Humans;  Hydrocortisone;  Male;  Middle Aged;  Prolactin;  Sepsis;  Stress, Physiological;  Systemic Inflammatory Response Syndrome
TODO - Objective. To evaluate the early heat shock protein (HSP) and hormonal stress response of intensive care unit (ICU) patients with severe sepsis/septic shock (SS) or systemic inflammatory response syndrome (SIRS) compared to healthy subjects (H). Methods. Patients with early (first 48 hrs) SS (n = 29) or SIRS (n = 29) admitted to a university ICU and 16 H were enrolled in the study. Serum prolactin, cortisol, and plasma ACTH were determined using immunoassay analyzers. ELISA was used to evaluate extracellular HSPs (eHSP90α, eHSP72) and interleukins. Mean fluorescence intensity (MFI) values for intracellular HSPs (iHSP72, iHSP90α) were measured using 4-colour flow-cytometry. Results. Prolactin, cortisol, and eHSP90α levels were significantly increased in SS patients compared to SIRS and H (P < 0.003). ACTH and eHSP72 were significantly higher in SS and SIRS compared to H (P < 0.005). SS monocytes expressed lower iHSP72 MFI levels compared to H (P = 0.03). Prolactin was related with SAPS III and APACHE II scores and cortisol with eHSP90α, IL-6, and lactate (P < 0.05). In SS and SIRS eHSP90α was related with eHSP72, IL-6, and IL-10. Conclusion. Prolactin, apart from cortisol, may have a role in the acute stress response in severe sepsis. In this early-onset inflammatory process, cortisol relates to eHSP90α, monocytes suppress iHSP72, and plasma eHSP72 increases. © 2014 K. Vardas et al.
ER -