TY - JOUR
TI - The high molecular weight glutenin subunit Bx7 allergen from wheat contains repetitive IgE epitopes
AU - Baar, A.
AU - Pahr, S.
AU - Constantin, C.
AU - Giavi, S.
AU - Papadopoulos, N.G.
AU - Pelkonen, A.S.
AU - Mäkelä, M.J.
AU - Scheiblhofer, S.
AU - Thalhamer, J.
AU - Weber, M.
AU - Ebner, C.
AU - Mari, A.
AU - Vrtala, S.
AU - Valenta, R.
JO - Allergy: European Journal of Allergy and Clinical Immunology
PY - 2014
VL - 69
TODO - 10
SP - 1316-1323
PB - Wiley-Blackwell Publishing Ltd
SN - null
TODO - 10.1111/all.12464
TODO - complementary DNA;  epitope;  food allergen;  glutenin;  high molecular weight glutenin subunit Bx7;  immunoglobulin E antibody;  unclassified drug;  allergen;  gluten;  glutenin;  immunoglobulin E;  recombinant protein, animal tissue;  Article;  bacteriophage lambda;  basophil degranulation;  degranulation assay;  DNA library;  effector cell;  food allergy;  human;  molecular cloning;  molecular weight;  nonhuman;  nucleotide sequence;  protein cross linking;  protein expression;  rat;  wheat;  amino acid sequence;  basophil degranulation test;  chemistry;  enzyme linked immunosorbent assay;  immunology;  molecular genetics;  wheat allergy, Allergens;  Amino Acid Sequence;  Basophil Degranulation Test;  Enzyme-Linked Immunosorbent Assay;  Epitopes, B-Lymphocyte;  Glutens;  Humans;  Immunoglobulin E;  Molecular Sequence Data;  Recombinant Proteins;  Wheat Hypersensitivity
TODO - Background: Wheat is one of the most common food allergen sources for children and adults. The aim of this study was to characterize new wheat allergens using an IgE discovery approach and to investigate their IgE epitopes.
Methods: A cDNA expression library representing the wheat transcriptome was constructed in phage lambda gt11 and screened with IgE antibodies from wheat food allergic patients. IgE-reactive cDNA clones coding for portions of high molecular weight (HMW) glutenin subunits were identified by sequence analysis of positive clones. IgE epitopes were characterized using recombinant fragments from the HMW Bx7 and synthetic peptides thereof for testing of allergic patients' sera and in basophil degranulation assays.
Results: We found that the major IgE-reactive areas of HMW glutenins are located in the repetitive regions of the protein and could show that two independent IgE-reactive fragments from HMW Bx7 contained repetitive IgE epitopes.
Conclusions: Our results demonstrate that IgE antibodies from wheat food allergic patients can recognize repetitive epitopes in one of the important wheat food allergens. Recombinant HMW Bx7 may be included into the panel of allergens for component-resolved diagnosis of wheat food allergy. © 2014 John Wiley & Sons A/S.
ER -