TY - JOUR
TI - Frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in Greece (2007-2008)
AU - Koukou, D.
AU - Grivea, I.
AU - Roma, E.
AU - Tsioni, H.
AU - Trimis, G.
AU - Galanakis, E.
AU - Farmaki, E.
AU - Iosifidis, E.
AU - Michos, A.
AU - Siamopoulou-Mavridou, A.
AU - Kalmanti, M.
AU - Papadopoulou, H.
AU - Roilides, E.
AU - Theodoridou, M.
AU - Syrogiannopoulos, G.A.
AU - Syriopoulou, V.
JO - Journal of Medical Virology
PY - 2011
VL - 83
TODO - 1
SP - 165-169
PB - 
SN - 0146-6615, 1096-9071
TODO - 10.1002/jmv.21945
TODO - virus antigen, acute gastroenteritis;  article;  child;  chromatography;  clinical feature;  clinical trial;  controlled clinical trial;  controlled study;  disease severity;  feces;  feces analysis;  female;  gastroenteritis;  gene sequence;  genotype;  Greece;  human;  infant;  major clinical study;  male;  multicenter study;  nonhuman;  pediatric hospital;  preschool child;  prevalence;  prospective study;  reverse transcription polymerase chain reaction;  Rotavirus;  species distribution, Antigens, Viral;  Child, Preschool;  Feces;  Female;  Gastroenteritis;  Genotype;  Greece;  Humans;  Infant;  Male;  Prevalence;  Prospective Studies;  Reverse Transcriptase Polymerase Chain Reaction;  RNA, Viral;  Rotavirus;  Rotavirus Infections;  Sequence Analysis, DNA, Rotavirus;  Rotavirus A
TODO - Rotavirus is the leading cause of acute gastroenteritis among young children worldwide. A prospective multi-center study was conducted (2007-2008) in five Pediatric Hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in Greece. Faecal samples were examined for the presence of group A rotavirus antigen by immunochromatography. Rotavirus strains were subjected to G and P genotyping by reverse-transcriptase polymerase chain reaction (PCR) and sequencing. A total of 393 children (216 boys) of median age 23 months, participated in the study. Rotavirus was the cause of acute gastroenteritis in 166 children, 42.3% (CI 95%, 37.4-47.1%) of non-hospitalized and 47.8% (CI 95%, 41.7-53.9%) of hospitalized patients. Rotavirus gastroenteritis occurred between December and April in 78.6% of the cases. Most children with RVG (77.8%) were between 3 months and 3 years old. The mean value of Clark severity score was 12.9 ± 5.1 for RVG and 10.5 ± 4.9 for non-RVG (P < 0.01). Genotypes were determined in 117 strains and their distribution was as following: G1P[8], 49%; G2P[4], 31%; G4P[8], 10%; G9P[8], 9%; and G8P[14], 1%. In conclusion, rotavirus is a frequent cause of acute gastroenteritis in Greece. The genotypes circulating are similar with those of other European countries. Copyright © 2010 Wiley-Liss, Inc.
ER -