TY - JOUR
TI - Visceral white adipose tissue and serum proteomic alternations in metabolically healthy obese patients undergoing bariatric surgery
AU - Doulamis, I.P.
AU - Konstantopoulos, P.
AU - Tzani, A.
AU - Antoranz, A.
AU - Minia, A.
AU - Daskalopoulou, A.
AU - Charalampopoulos, A.
AU - Alexopoulos, L.
AU - Perrea, D.N.
AU - Menenakos, E.
JO - Cytokine
PY - 2019
VL - 115
TODO - null
SP - 76-83
PB - INSTAP Academic Press
SN - 1043-4666, 1096-0023
TODO - 10.1016/j.cyto.2018.11.017
TODO - carboxy terminal telopeptide;  endocrine gland derived vascular endothelial growth factor;  gelatinase B;  growth differentiation factor 15;  interleukin 20;  macrophage inflammatory protein 1alpha;  resistin;  tumor necrosis factor;  tumor necrosis factor related apoptosis inducing ligand;  tumor necrosis factor weak inducer of apoptosis;  unclassified drug;  biological marker;  cytokine;  proteome, adult;  Article;  biliopancreatic bypass;  body mass;  clinical article;  comorbidity;  controlled study;  enzyme repression;  female;  human;  human tissue;  intra-abdominal white adipose tissue;  laparoscopic sleeve gastrectomy;  male;  obesity;  population research;  priority journal;  prospective study;  protein blood level;  protein expression;  proteomics;  Roux-en-Y gastric bypass;  upregulation;  bariatric surgery;  metabolism;  morbid obesity;  obesity;  physiology;  procedures;  proteomics;  white adipose tissue, Adipose Tissue, White;  Adiposity;  Adult;  Bariatric Surgery;  Biomarkers;  Cytokines;  Female;  Humans;  Male;  Obesity, Morbid;  Proteome;  Proteomics
TODO - Metabolically healthy obesity is characterized as a comorbidity-free obesity status, however the exact pathogenetic mechanisms implicated in its transition to unhealthy obesity have not yet been unveiled. Our aim was to investigate the effect of metabolic health on the proteomic profile both in serum and visceral fat of morbidly obese subjects. 28 patients undergoing bariatric surgery were prospectively enrolled. They were divided into two groups: metabolically healthy (MHO, n = 18) and unhealthy (MUO, n = 10) obese patients. 30 biomarkers were measured in serum and visceral adipose tissue with the use of targeted proteomic analysis (Luminex assays). TNF weak inducer of apoptosis (TWEAK) (p = 0.043), TNF related apoptosis inducing ligand (TRAIL) (p = 0.037), Growth differentiation factor-15 (GDF-15) (p = 0.04), Resistin (RETN) (p = 0.047), Matrix metalloproteinase-9 (MMP-9) (p = 0.011) and C-terminal telopeptide (ICTP) (p = 0.022) were up-regulated in the MUO group in the visceral white adipose tissue. Moreover, C-C motif ligand-3 (CCL-3) (p = 0.056), Interleukin-20 (IL-20) (p = 0.04), Prokineticin-1 (PROK-1) (p = 0.028) and TWEAK (p = 0.016) were found to be suppressed in the serum of MHO group. Significant correlations between serum and adipose tissue levels of certain cytokines were also observed, while 16 biomarkers were associated with BMI. Our results indicate metabolic health substantially attenuates the expression of TWEAK, TRAIL, GDF-15, RETN, MMP-9 and ICTP expression locally, in the visceral white adipose tissue, and the expression of CCL-3, IL-20, PROK-1 and TWEAK in the peripheral blood. Intriguingly, different cytokines –except for TWEAK- are up-regulated in each site, suggesting that obesity is not a homogenous but a multi-dimensional disease. © 2018 Elsevier Ltd
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