TY - JOUR
TI - Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus
AU - Logotheti, M.
AU - Kotsovili-Tseleni, A.
AU - Arsenis, G.
AU - Legakis, N.I.
JO - Journal of Microbiological Methods
PY - 2009
VL - 76
TODO - 2
SP - 209-211
PB - 
SN - 0167-7012
TODO - 10.1016/j.mimet.2008.10.006
TODO - aspergillopepsin;  pepsin A;  ribosome DNA;  unclassified drug, amplicon;  animal experiment;  article;  Aspergillus flavus;  Aspergillus fumigatus;  Aspergillus niger;  Aspergillus terreus;  discriminant analysis;  fungal strain;  gene sequence;  human;  human experiment;  multiplex polymerase chain reaction;  nonhuman;  normal human;  pathogenesis;  priority journal, Aspergillosis;  Aspergillus flavus;  Aspergillus fumigatus;  Aspergillus niger;  DNA Primers;  DNA, Fungal;  Humans;  Mycological Typing Techniques;  Polymerase Chain Reaction;  Sensitivity and Specificity, Aspergillus;  Aspergillus flavus;  Aspergillus fumigatus;  Aspergillus niger;  Aspergillus terreus
TODO - Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species. © 2008 Elsevier B.V. All rights reserved.
ER -