TY - JOUR
TI - Phylogenetic and antigenic characterization of new fish nodavirus isolates from Europe and Asia
AU - Skliris, G.P.
AU - Krondiris, J.V.
AU - Sideris, D.C.
AU - Shinn, A.P.
AU - Starkey, W.G.
AU - Richards, R.H.
JO - Virus Research
PY - 2001
VL - 75
TODO - 1
SP - 59-67
PB - 
SN - 0168-1702
TODO - 10.1016/S0168-1702(01)00225-8
TODO - coat protein, amino acid sequence;  antigenicity;  article;  Asia;  Europe;  fish;  genetic analysis;  genotype;  Nodavirus;  nucleotide sequence;  phylogeny;  priority journal;  sequence analysis;  serology;  species difference;  virus characterization;  virus genome;  virus isolation;  virus neutralization, Amino Acid Sequence;  Animals;  Antigens, Viral;  Asia;  Capsid;  Cloning, Molecular;  Europe;  Fishes;  Genome, Viral;  Genotype;  Immune Sera;  Molecular Sequence Data;  Neutralization Tests;  Phylogeny;  Rabbits;  RNA Viruses;  Sequence Alignment, Nodaviridae;  Redspotted grouper nervous necrosis virus;  Striped Jack nervous necrosis virus
TODO - Nodaviruses are widespread causative agents of viral nervous necrosis in fish. Based on the coat protein sequence, fish nodaviruses are categorized into four different genotypes. In this study, we present data on the phylogenetic and antigenic characterization of 12 new isolates, eight European and four of Asian origin, from farmed and wild species of fish. Phylogenetic analysis based on the nucleotide sequence (688 bases) of the coat protein classified the majority of these new isolates to the RGNNV genotype. Geographic or host-species specificities were not revealed by this study. Neutralizing assay experiments, further confirmed the genotypic classification, supporting the possibility that the different nodavirus genotypes can also be serologically distinguishable. © 2001 Elsevier Science B.V.
ER -