TY - JOUR
TI - Effects of pulsed electric fields on DNA of human lymphocytes
AU - Delimaris, J.
AU - Tsilimigaki, S.
AU - Messini-Nicolaki, N.
AU - Ziros, E.
AU - Piperakis, S.M.
JO - Cell Biology and Toxicology
PY - 2006
VL - 22
TODO - 6
SP - 409-415
PB - 
SN - 0742-2091, 1573-6822
TODO - 10.1007/s10565-006-0105-1
TODO - DNA;  hydrogen peroxide, adult;  article;  comet assay;  controlled study;  DNA damage;  DNA repair;  exposure;  gamma irradiation;  genotoxicity;  human;  human cell;  human experiment;  incubation temperature;  lymphocyte;  male;  priority journal;  productivity;  pulsed electric field, Adult;  Comet Assay;  DNA;  DNA Damage;  DNA Repair;  Dose-Response Relationship, Radiation;  Electromagnetic Fields;  Gamma Rays;  Humans;  Hydrogen Peroxide;  Lymphocytes;  Male;  Middle Aged;  Oxidants
TODO - The effects of pulsed electric fields of low frequency (50 Hz) on DNA of human lymphocytes were investigated. The influence of additional external factors, such as hydrogen peroxide (H2O2) and γ-irradiation, as well as the repair efficiency in these lymphocytes, was also evaluated. The comet assay, a very sensitive and rapid method for detecting DNA damage at the single cells level was the method used. A significant amount of damage was observed after exposure to the electric fields, compared to the controls. After 2 h incubation at 37°C, a proportion of damage was repaired. H2O2 and γ-irradiation increased the damage to lymphocytes exposed to pulsed electric fields according to the dose used, while the amount of the repair was proportional to the damage. © 2006 Springer Science + Business Media, Inc.
ER -