TY - JOUR
TI - Molecular and immunological characterization of Mus a 5 allergen from banana fruit
AU - Aleksic, I.
AU - Popovic, M.
AU - Dimitrijevic, R.
AU - Andjelkovic, U.
AU - Vassilopoulou, E.
AU - Sinaniotis, A.
AU - Atanaskovic-Markovic, M.
AU - Lindner, B.
AU - Petersen, A.
AU - Papadopoulos, N.G.
AU - Gavrovic-Jankulovic, M.
JO - Molecular Nutrition and Food Research
PY - 2012
VL - 56
TODO - 3
SP - 446-453
PB - 
SN - null
TODO - 10.1002/mnfr.201100541
TODO - allergen;  CD63 antigen;  CD63 protein, human;  endo 1,3(4) beta glucanase;  ENPP3 protein, human;  immunoglobulin E;  inorganic pyrophosphatase;  isoprotein;  phosphodiesterase;  plant antigen;  vegetable protein, adolescent;  adult;  amino acid sequence;  article;  chemistry;  female;  food allergy;  fruit;  genetics;  human;  immunology;  isolation and purification;  male;  metabolism;  methodology;  molecular cloning;  molecular genetics;  Musa;  skin test;  upregulation, Adolescent;  Adult;  Allergens;  Amino Acid Sequence;  Antigens, CD63;  Antigens, Plant;  Cloning, Molecular;  Endo-1,3(4)-beta-Glucanase;  Female;  Food Hypersensitivity;  Fruit;  Humans;  Immunoglobulin E;  Male;  Molecular Sequence Data;  Musa;  Phosphoric Diester Hydrolases;  Plant Proteins;  Protein Isoforms;  Pyrophosphatases;  Skin Tests;  Up-Regulation;  Young Adult, Musa acuminata
TODO - Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner Conclusion Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
ER -