TY - JOUR
TI - Optimization of psoriasis mouse models
AU - Karamani, C.
AU - Antoniadou, I.T.
AU - Dimou, A.
AU - Andreou, E.
AU - Kostakis, G.
AU - Sideri, A.
AU - Vitsos, A.
AU - Gkavanozi, A.
AU - Sfiniadakis, I.
AU - Skaltsa, H.
AU - Papaioannou, G.T.
AU - Rallis, M.C.
AU - Maibach, H.
JO - Journal of Pharmacological and Toxicological Methods
PY - 2021
VL - 108
TODO - null
SP - null
PB - ELSEVIER SCIENCE INC 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
SN - 1056-8719
TODO - 10.1016/j.vascn.2021.107054
TODO - acetic acid;  antidepressant agent;  anxiolytic agent;  drinking water;  imiquimod;  imiquimod, animal cell;  animal experiment;  animal model;  animal tissue;  apolipoprotein E knockout mouse;  Article;  Bagg albino mouse;  behavior assessment;  biophysics;  C57BL 6 mouse;  cell infiltration;  controlled study;  cross breeding;  disease model;  dry skin;  epidermal thickness;  erythema;  histopathology;  hyperkeratosis;  immobility;  inflammatory cell;  locomotion;  male;  mouse;  nonhuman;  parakeratosis;  phenotype;  process optimization;  psoriasis;  Psoriasis Area and Severity Index;  rearing;  skin abscess;  skin water loss;  animal;  C57BL mouse;  disease model;  psoriasis;  skin, Animals;  Disease Models, Animal;  Imiquimod;  Mice;  Mice, Inbred BALB C;  Mice, Inbred C57BL;  Psoriasis;  Skin
TODO - Introduction: Psoriasis, is a common, chronic, autoimmune, inflammatory, relapsing disease, which would benefit from reliable and human-relevant animal models to test drugs pre-clinically and to understand their mechanism of action. Because of its ease of use, convenience and low cost, the imiquimod (IMQ)-induced psoriasis-like model is widely utilized; however, it is not known whether all mouse strains are equivalent and if the hairless mouse is appropriate, so that the imiquimod model can be further optimized. Methods: Under similar experimental conditions, common mouse strains (BALB/c, C57BL/6J, and ApoE) and a new hairless strain (ApoE/SKH-hr2) as well as several inducers (IMQ, IMQ + acetic acid (AcOH) topical and IMQ + AcOH systemic) were compared by clinical, histopathological, biophysical and locomotor activity assessments. Results and discussion: The BALB/c mice yielded an optimal psoriasis-like phenotype with IMQ + AcOH topical treatment, and the corresponding phenotypes for the other mouse strains were C57BL/6J moderate and ApoE mild. In contrast, the ApoE/SKH-hr2 mice, as a result of the absence of a Munro abscess in the histopathology analysis, left doubt about the psoriasis-like acquisition. Locomotor activity of BALB/c mice treated with IMQ, IMQ + AcOH topically and IMQ + AcOH systemically showed decreased distance and rearing coverage and increased immobility with all treatments. Hence, the BALB/c mouse strain appears to be an optimal psoriasis-like model when utilizing IMQ + AcOH topical application. © 2021 Elsevier Inc.
ER -