TY - JOUR
TI - Impact of deserosalization on small bowel anastomosis healing in swine: A pilot study
AU - Vrakopoulou, G.-Z.
AU - Toutouzas, K.G.
AU - Giannios, P.
AU - Panousopoulos, S.-G.
AU - Theodoropoulos, C.
AU - Danas, E.
AU - Liakea, A.
AU - Papalois, A.E.
AU - Zografos, G.
AU - Larentzakis, A.
JO - In vivo (Athens, Greece)
PY - 2020
VL - 34
TODO - 5
SP - 2423-2429
PB - International Institute of Anticancer Research
SN - null
TODO - 10.21873/invivo.12056
TODO - hydroxyproline;  collagen, animal experiment;  animal model;  animal tissue;  Article;  chemical reaction;  controlled study;  deserosalization;  feasibility study;  female;  histopathology;  intestine anastomosis;  jejuno jejunal side to side anastomosis;  jejunum;  Landrace pig;  nonhuman;  pilot study;  serosa;  statistically significant result;  wound healing;  anastomosis;  animal;  colon;  pig;  small intestine;  surgery, Anastomosis, Surgical;  Animals;  Collagen;  Colon;  Intestine, Small;  Pilot Projects;  Swine;  Wound Healing
TODO - Background: Healing is related to gastrointestinal anastomotic leak, which is a severe and common complication. This study aimed to investigate the feasibility and the impact of deserosalization on healing of jejuno-jejunal anastomoses in an animal model. Materials and Methods: Seven swine underwent three types of side-to-side jejuno-jejunal anastomosis twice and survived seven days. Three different types of jejuno-jejunal sideto- side anastomoses were performed twice at 20-cm distance from each other in each animal: no serosa removal, one-sided, and two-sided serosa removal, respectively. Bursting pressure, tissue hydroxyproline concentration, and pathology scores were evaluated. Results: Hydroxyproline tissue concentration was a mean±standard deviation of 0.37±0.09, 0.38±0.08, and 0.30±0.05 nmoI/ml respectively (p<0.05). Bursting pressure was a mean±standard deviation of 59.02±8.60, 73.20±11.09, and 100.01±7.49 mmHg, respectively (p<0.001). The histopathological assessment did not find any statistically significant differences. Conclusion: Deserosalization in jejunojejunal anastomosis was technically feasible and seemed to improve mechanical strength and collagen deposition in this experimental porcine model. Further investigation is warranted. © 2020 International Institute of Anticancer Research. All rights reserved.
ER -