TY - JOUR
TI - Deep sequencing identified dysregulated circulating MicroRNAs in late onset Preeclampsia
AU - Mavreli, D.
AU - Lykoudi, A.
AU - Lambrou, G.
AU - Papaioannou, G.
AU - Vrachnis, N.
AU - Kalantaridou, S.
AU - Papantoniou, N.
AU - Kolialexi, A.
JO - In vivo (Athens, Greece)
PY - 2020
VL - 34
TODO - 5
SP - 2317-2324
PB - International Institute of Anticancer Research
SN - null
TODO - 10.21873/invivo.12044
TODO - circulating microRNA;  glucagon;  glycosaminoglycan;  insulin;  microRNA 23b 5p;  microRNA 99b 5p;  T lymphocyte receptor;  unclassified drug;  biological marker;  circulating microRNA;  microRNA, adult;  Article;  case control study;  clinical article;  controlled study;  down regulation;  female;  first trimester pregnancy;  gene ontology;  high throughput sequencing;  human;  human tissue;  immune response;  insulin resistance;  insulin signaling;  maternal plasma;  osteoclastogenesis;  preeclampsia;  pregnancy outcome;  real time polymerase chain reaction;  retrospective study;  RNA sequencing;  upregulation;  gene expression profiling;  genetics;  high throughput sequencing;  preeclampsia;  pregnancy, Biomarkers;  Circulating MicroRNA;  Female;  Gene Expression Profiling;  High-Throughput Nucleotide Sequencing;  Humans;  MicroRNAs;  Pre-Eclampsia;  Pregnancy
TODO - Background/Aim: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop lateonset preeclampsia (LOPE) compared to uncomplicated pregnancies. Materials and Methods: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. Results: miR-23b-5p and miR- 99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. Conclusion: miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate noninvasive biomarkers for early prediction and prevention. © 2020 International Institute of Anticancer Research. All rights reserved.
ER -