TY - JOUR
TI - Isolation and antimicrobial activity of coumarin derivatives from fruits of peucedanum luxurians tamamsch
AU - Widelski, J.
AU - Luca, S.V.
AU - Skiba, A.
AU - Chinou, I.
AU - Marcourt, L.
AU - Wolfender, J.-L.
AU - Skalicka-Wozniak, K.
JO - Molecules (Basel, Switzerland)
PY - 2018
VL - 23
TODO - 5
SP - null
PB - MDPI AG
SN - null
TODO - 10.3390/molecules23051222
TODO - antiinfective agent;  coumarin derivative;  plant extract, aerial plant part;  Apiaceae;  chemical structure;  chemistry;  counter current chromatography;  drug effect;  fruit;  Gram negative bacterium;  Gram positive bacterium;  isolation and purification;  microbial sensitivity test, Anti-Infective Agents;  Apiaceae;  Coumarins;  Countercurrent Distribution;  Fruit;  Gram-Negative Bacteria;  Gram-Positive Bacteria;  Microbial Sensitivity Tests;  Molecular Structure;  Plant Components, Aerial;  Plant Extracts
TODO - As a continuation of searching for phytoconstituents that act as promising agents for antimicrobial therapy, rare coumarins were isolated from fruits of Peucedanum luxurians and tested. In a first step, the content of major compounds in the aerial parts and fruits of P. luxurians were compared. The results clearly showed that the fruits with dichloromethane as a solvent yielded, in most cases, higher concentrations of almost all the analyzed coumarins than the aerial parts, with peucedanin detected as the most abundant compound with a concentration of 4563.94 ± 3.35 mg/ 100 g. Under this perspective, the dichloromethane extract from the fruits of P. luxurians was further submitted to high performance countercurrent chromatography with a mixture of n-hexane, ethyl acetate, methanol, and water 6:5:6:5 (v/v). Combination of HPCCC and prep-HPLC yielded 6,7-dihydroxybergamottin (1), officinalin (2), stenocarpin isobutyrate (3), officinalin isobutyrate (4), 8-methoxypeucedanin (5), and peucedanin (6). Isolated compounds were tested against several Gram-positive and Gram-negative bacteria strains. 6,7-Dihydroxybergamottin, peucedanin, and officinalin isobutyrate appeared to be the most active against all tested bacteria strains with minimum inhibitory concentration (MIC) values between 1.20 and 4.80 mg/mL. To the best of our knowledge, this is the first report about countercurrent isolation of mentioned coumarins, as well as the first information about their antimicrobial activity. © 2018 by the authors.
ER -