TY - JOUR
TI - Evaluation of cadmium-induced transcriptome alterations by three color cDNA labeling microarray analysis on a T-cell line
AU - Tsangaris, G.Th.
AU - Botsonis, A.
AU - Politis, I.
AU - Tzortzatou-Stathopoulou, F.
JO - EMC - Toxicologie-Pathologie
PY - 2002
VL - 178
TODO - 2
SP - 135-160
PB - 
SN - null
TODO - 10.1016/S0300-483X(02)00236-6
TODO - cadmium;  complementary DNA;  fluorescent dye;  RNA, animal cell culture;  apoptosis;  article;  biotransformation;  cell death;  cell function;  cell line;  controlled study;  DNA determination;  DNA microarray;  down regulation;  evaluation;  human;  human cell;  labeling index;  nucleotide sequence;  priority journal;  RNA extraction;  T lymphocyte;  unindexed sequence, Apoptosis;  Blotting, Northern;  Cadmium;  Cell Survival;  Culture Media;  DNA, Complementary;  Fluorescent Dyes;  Immunohistochemistry;  In Situ Hybridization, Fluorescence;  Indicators and Reagents;  Lasers;  Mutagens;  Oligonucleotide Array Sequence Analysis;  Reverse Transcriptase Polymerase Chain Reaction;  RNA, Messenger;  T-Lymphocytes;  Up-Regulation
TODO - Beside heavy metals, cadmium (Cd2+) is a ubiquitous toxic metal with a well established apoptotic and genotoxic effect, chronic exposure of which has been involved in a variety of pathological conditions. In the present study, we investigated by 1455 genes cDNA microarrays the toxic and apoptotic effect of Cd2+, on the T-cell line CCRF-CEM, applying a three laser differential analysis, on the same microarray slide. The cells were cultured for 6 and 24 h in the absence (control) or presence of Cd2+ (10 or 20 μM), RNAs were extracted and the produced cDNAs were labeled with rhodamine derivatives fluorescent dyes. A microarray slide was simultaneously hybridized by the labeled cDNAs and analyzed. We found that, in relation to control, treatment of the cells for 6 h with 10 and 20 μM Cd2+, induces up-regulation in 20 and 34 genes, respectively. Treatment for 24 h with 10 and 20 μM Cd2+ induces up-regulation in 22 and 84 genes, respectively. Twenty-eight genes were found down-regulated only after treatment for 24 h with Cd2+ 10 μM. These data suggest that Cd2+ produces a time- and dose-dependent molecular cascade, induces disturbances in different subcellular compartments, influencing thereafter the normal cellular functions, the differentiation process, the malignant transformation and the cell death. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
ER -