TY - JOUR TI - The effects of light on callus growth and somatic embryogenesis from Lavandula vera and Teucrium chamaedrys: A preliminary study AU - Kintzios, S. AU - Papanastasiou, I. AU - Tourgelis, P. AU - Papastellatos, C. AU - Georgopoulos, V. AU - Drossopoulos, J. JO - Journal of Herbs, Spices and Medicinal Plants PY - 2002 VL - 9 TODO - 2-3 SP - 223-227 PB - Taylor and Francis Inc. SN - null TODO - 10.1300/J044v09n02_32 TODO - boron; kinetin, embryonic development; light, conference paper; explant; illumination; incubation time; Lavandula vera; light irradiance; Murashige and Skoog medium; nonhuman; photoperiodicity; plant; plant growth; somatic embryogenesis; Teucrium chamaedrys; tissue culture, Lavandula angustifolia; Lavandula vera; Teucrium; Teucrium chamaedrys; Teucrium chamaedrys TODO - In order to investigate the effect of light on the tissue culture of lavender (Lavandula vera L.) and wall germander (Teucrium chamaedrys L.), leaf explants from greenhouse-grown plants were inoculated on a Murashige and Skoog (MS) medium (1) supplemented with 1 mg L-1 2,4-D and 2 mg L-1 kinetin and incubated either (i) continuously in the darkness for 6 weeks or (ii) first in the darkness for 0-5 weeks and then under illumination (150 μmol m-2 s-1, 16 hour light/8 hour dark photoperiod). Lavender callus growth was improved under increased incubation in darkness while somatic embryogenesis was remarkably reduced under the same conditions. Incubation in darkness did not affect the growth of wall germander cultures, but improved somatic embryogenesis. Moreover, a possible participation of boron in the effect of light on somatic embryogenesis for both species was indicated. © 2002 by The Haworth Press, Inc. All rights reserved. ER -