TY - JOUR
TI - PreDicta chip-based high resolution diagnosis of rhinovirus-induced wheeze
AU - Niespodziana, K.
AU - Stenberg-Hammar, K.
AU - Megremis, S.
AU - Cabauatan, C.R.
AU - Napora-Wijata, K.
AU - Vacal, P.C.
AU - Gallerano, D.
AU - Lupinek, C.
AU - Ebner, D.
AU - Schlederer, T.
AU - Harwanegg, C.
AU - Söderhäll, C.
AU - Van Hage, M.
AU - Hedlin, G.
AU - Papadopoulos, N.G.
AU - Valenta, R.
JO - Nature Communications
PY - 2018
VL - 9
TODO - 1
SP - null
PB - Nature Publishing Group
SN - 2041-1723
TODO - 10.1038/s41467-018-04591-0
TODO - peptide;  unclassified drug;  viral protein;  virus peptide;  viral protein 1, rhinovirus, asthma;  chronic obstructive pulmonary disease;  disease treatment;  induced response;  peptide;  protein;  resolution;  symptom;  viral disease, adolescent;  adult;  Article;  blood sampling;  child;  female;  human;  Human rhinovirus A;  Human rhinovirus C;  major clinical study;  male;  microarray analysis;  nonhuman;  Rhinovirus;  Rhinovirus infection;  virus strain;  wheezing;  abnormal respiratory sound;  asthma;  classification;  clinical trial;  devices;  immunology;  infant;  isolation and purification;  picornavirus infection;  preschool child;  procedures;  protein microarray;  serology;  virology, Rhinovirus, Asthma;  Child, Preschool;  Female;  Humans;  Infant;  Male;  Picornaviridae Infections;  Protein Array Analysis;  Respiratory Sounds;  Rhinovirus;  Serologic Tests;  Viral Proteins
TODO - Rhinovirus (RV) infections are major triggers of acute exacerbations of severe respiratory diseases such as pre-school wheeze, asthma and chronic obstructive pulmonary disease (COPD). The occurrence of numerous RV types is a major challenge for the identification of the culprit virus types and for the improvement of virus type-specific treatment strategies. Here, we develop a chip containing 130 different micro-arrayed RV proteins and peptides and demonstrate in a cohort of 120 pre-school children, most of whom had been hospitalized due to acute wheeze, that it is possible to determine the culprit RV species with a minute blood sample by serology. Importantly, we identify RV-A and RV-C species as giving rise to most severe respiratory symptoms. Thus, we have generated a chip for the serological identification of RV-induced respiratory illness which should be useful for the rational development of preventive and therapeutic strategies targeting the most important RV types. © 2018 The Author(s).
ER -