TY - JOUR
TI - Doing synthetic biology with photosynthetic microorganisms
AU - Vavitsas, Konstantinos
AU - Kugler, Amit
AU - Satta, Alessandro and
AU - Hatzinikolaou, Dimitris G.
AU - Lindblad, Peter
AU - Fewer, David P. and
AU - Lindberg, Pia
AU - Toivari, Mervi
AU - Stensjo, Karin
JO - Physiologia Plantarum
PY - 2021
VL - 173
TODO - 2, SI
SP - 624-638
PB - Wiley
SN - 0031-9317, 1399-3054
TODO - 10.1111/ppl.13455
TODO - null
TODO - The use of photosynthetic microbes as synthetic biology hosts for the
sustainable production of commodity chemicals and even fuels has
received increasing attention over the last decade. The number of
studies published, tools implemented, and resources made available for
microalgae have increased beyond expectations during the last few years.
However, the tools available for genetic engineering in these organisms
still lag those available for the more commonly used heterotrophic host
organisms. In this mini-review, we provide an overview of the
photosynthetic microbes most commonly used in synthetic biology studies,
namely cyanobacteria, chlorophytes, eustigmatophytes and diatoms. We
provide basic information on the techniques and tools available for each
model group of organisms, we outline the state-of-the-art, and we list
the synthetic biology tools that have been successfully used. We
specifically focus on the latest CRISPR developments, as we believe that
precision editing and advanced genetic engineering tools will be pivotal
to the advancement of the field. Finally, we discuss the relative
strengths and weaknesses of each group of organisms and examine the
challenges that need to be overcome to achieve their synthetic biology
potential.
ER -