TY - JOUR
TI - SARS-CoV-2 seroepidemiological study in healthcare workers and
discordant results using seven different diagnostic methods
AU - Tatsi, Elizabeth-Barbara
AU - Dellis, Charilaos
AU - Petridou, Evangelia
AU - and Banou, Kirkira
AU - Zachariadou, Levantia
AU - Syriopoulou, Vassiliki
AU - and Michos, Athanasios
JO - Healthcare Infection
PY - 2022
VL - 50
TODO - 1
SP - 251-256
PB - Springer Berlin Heidelberg
SN - null
TODO - 10.1007/s15010-021-01653-2
TODO - SARS-CoV-2; COVID-19; Serology; Antibody; Comparison; Immunity
TODO - The aim of the study was to access the SARS-CoV-2 antibody
seroprevalence in healthcare workers (HCWs) of a tertiary pediatric
hospital after the first wave of the pandemic and to compare the results
among seven commercially available antibody detection assays, including
chemiluminescence (CMIA), electroluminescence (ECLIA), Epsilon
nzyme-Linked Immunosorbent Assay (ELISA), and rapid immunochromatography
(RIC). SARS-CoV-2 antibody detection was performed in serum samples of
1216 HCWs, using a reference CMIA assay and 8/1216 (0.66%) were
detected positive. Positive serum samples were further tested with other
assays; however, only one sample was positive by all tests. The rest 7
cases were negative with ECLIA and ELISA and gave discordant results
with RIC test. Six months later, new serum samples of seropositive HCWs
were analyzed with the same 7 tests, with inconsistent results again.
Identification of reliable SARS-CoV-2 antibody tests is important to
determine the actual number of past infections, the duration of
antibodies, and guide public health decisions.
ER -