TY - JOUR
TI - Pneumococcal Serotype Identification by Capsular Sequence Typing (CST):
A Modified Novel Approach for Serotyping Directly in Clinical Samples
AU - Marmaras, Nektarios
AU - Xirogianni, Athanasia
AU - Papandreou, Anastasia
AU - and Petinaki, Efthymia
AU - Papaevangelou, Vana
AU - Tsolia, Maria and
AU - Tzanakaki, Georgina
JO - DIAGNOSTIC ONCOLOGY
PY - 2021
VL - 11
TODO - 12
SP - null
PB - MDPI
SN - null
TODO - 10.3390/diagnostics11122353
TODO - S; pneumoniae; non-culture serotyping; capsular sequence typing (CST)
TODO - As almost 60-70% of Invasive Pneumococcal Disease (IPD) is identified
by nonculture methods in Greece, serotyping is of high importance for
the better monitoring of pneumococcal serotypes due to the availability
of conjugate vaccines. The aim of the study was the modification and
direct application of the Capsular Sequence Typing (CST) assay in
clinical samples in order to serotype Streptococcus pneumoniae
culture-negative, Polymerase Chain Reaction (PCR_-positive samples,
followed by CST group specific single-tube PCR assays. A two-step PCR
modified assay was applied on a total of 306 samples (such as CSF,
blood, pleural and middle ear fluids, isolates) obtained from 283
patients with IPD. The overall performance permits a rapid, accurate and
cost-effective method for nonculture pneumococcal serotyping. As the
management of IPD is closely related to the continuous monitoring of
pneumococcal serotypes, the proposed approach proved to be a valuable
tool for the typing and epidemiological monitoring of S. pneumoniae, for
the evaluation of the overall impact of vaccination programs in the era
of pneumococcal conjugate vaccines, in order to initiate the appropriate
vaccination strategy.
ER -