TY - JOUR
TI - CHARACTERIZATION OF THE CHROMOSOMAL AAC(6')-IC GENE FROM
SERRATIA-MARCESCENS
AU - SHAW, KJ
AU - RATHER, PN
AU - SABATELLI, FJ
AU - MANN, P
AU - MUNAYYER, H
AU - and MIERZWA, R
AU - PETRIKKOS, GL
AU - HARE, RS
AU - MILLER, GH and
AU - BENNETT, P
AU - DOWNEY, P
JO - Antimicrobial Agents and Chemotherapy
PY - 1992
VL - 36
TODO - 7
SP - 1447-1455
PB - AMER SOC MICROBIOLOGY
SN - 0066-4804, 1098-6596
TODO - 10.1128/AAC.36.7.1447
TODO - null
TODO - The DNA sequence of the chromosomal aac(6’)-k gene from Serratia
marcescens, which had been previously cloned (H. M. Champion, P. M.
Bennett, D. A. Lewis, and D. S. Reeves, J. Antimicrob. Chemother.
22:587-596, 1988) was determined. High-pressure liquid chromatographic
analysis of extracts prepared from Escherichia coli carrying the
chromosomal aac(6’)-Ic gene on a plasmid confirmed the presence of
6’-N-acetyltransferase activity in this strain, which was suggested by
the aminoglycoside resistance profile. DNA sequence analysis of the
cloned 2,057-bp PstI fragment revealed several regions of homology to
previously characterized sequences from GenBank, including the rpoD and
tRNA-2 genes of E. coli. Subcloning experiments confirmed the coding
sequence of the aac(6’)-Ic gene to be at positions 1554 to 1992. The
predicted amino acid sequence of the AAC(6’)-Ic protein suggested that
it was the third member of a family of AAC(6’) proteins which included a
coding region identified between the aadB and aadA genes of Tn4000 and
an AAC(6’) protein encoded by pUO490, which was isolated from
Enterobacter cloacae. Primer extension analysis suggested that the -35
region of the aac(6’)-Ic promoter overlapped a large palindromic
sequence which may be involved in the regulation of the aac(6’)-Ic gene.
Hybridization experiments utilizing a restriction fragment from the
aac(6’)-Ic gene showed that all S. marcescens organisms carried this
gene whether or not the AAC(6’)-I resistance profile was expressed.
Organisms other than Serratia spp. did not hybridize to this probe.
ER -