TY - JOUR
TI - SIMULTANEOUS ASSESSMENT OF INSULIN-SECRETION AND INSULIN SENSITIVITY
USING A HYPERGLYCEMIC CLAMP
AU - MITRAKOU, A
AU - VUORINENMARKKOLA, H
AU - RAPTIS, G
AU - TOFT, I
AU - MOKAN,
AU - M
AU - STRUMPH, P
AU - PIMENTA, W
AU - VENEMAN, T
AU - JENSSEN, T
AU - BOLLI,
AU - G
AU - KORYTKOWSKI, M
AU - YKIJARVINEN, H
AU - GERICH, J
JO - JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
PY - 1992
VL - 75
TODO - 2
SP - 379-382
PB - ENDOCRINE SOC
SN - 0021-972X
TODO - 10.1210/jc.75.2.379
TODO - null
TODO - A hyperglycemic clamp is an established method to assess insulin
secretion and is generally used only for this purpose. To determine
whether it could also be used to assess insulin sensitivity, we compared
insulin sensitivity indices (ISI) obtained during euglycemic and
hyperglycemic clamp experiments in 22 nonobese volunteers (body mass
index, 23.9 +/- 0.6 kg/m2) and in 20 obese individuals (body mass index,
30.8 +/- 1.3 kg/M2) matched for age and gender. The ISI values
(micromoles per kg-min/pmol) of the obese group assessed during
hyperglycemic (0.088 +/- 0.011) and euglycemic (0.050 +/- 0.005) clamp
experiments were both significantly lower than the ISI of the nonobese
group assessed in hyperglycemic and euglycemic clamp experiments (0.179
+/- 0.024 and 0.096 +/- 0.009, respectively; both P < 0.01). Although
the ISI values obtained with hyperglycemic clamps were consistently
greater than those obtained with euglycemic clamp (0.137 +/- 0.016 vs.
0.075 +/- 0.007; P < 0.001), they were highly correlated (r = 0.84; P <
0.0001). Moreover, when these indices were converted to clearance rates,
thereby correcting for the mass action effects of glucose on glucose
disposal, the values obtained with the hyperglycemic clamp (0.0137 +/-
0.0016 mL/kg . min/pmol) were statistically identical to those obtained
with the euglycemic clamp (0.0142 +/- 0.0013 mL/kg . min/pmol), as
indicated by a regression equation having an intercept of 0 and a slope
(1.03) not different from 1. We, therefore, conclude that the
hyperglycemic clamp and the euglycemic clamp yield comparable estimates
of insulin sensitivity and that, under appropriate conditions, the
hyperglycemic clamp technique may be used to assess both insulin
sensitivity and insulin secretion in the same individual in a single
experiment.
ER -