TY - JOUR
TI - FUROSEMIDE-INDUCED HYPERGLYCEMIA - THE IMPLICATION OF GLYCOLYTIC KINASES
AU - DIMITRIADIS, G
AU - TEGOS, C
AU - GOLFINOPOULOU, L
AU - ROBOTI, C and
AU - RAPTIS, S
JO - Hormone and Metabolic Research
PY - 1993
VL - 25
TODO - 11
SP - 557-559
PB - Georg Thieme Verlag
SN - 0018-5043, 1439-4286
TODO - 10.1055/s-2007-1002176
TODO - FUROSEMIDE; MUSCLE; GLYCOLYSIS; GLUCOSE UTILIZATION
TODO - Hyperglycaemia is a well known adverse effect of therapy with diuretics.
In adipose tissue, hydrochlorothiazide and furosemide inhibit the rate
of glucose transport. In skeletal muscle, furosemide decreases the rate
of glucose phosphorylation and glycolysis. However, whether furosemide
has any direct effect on the activities of any of the glycolytic enzymes
is not known. In the present study, the effects of furosemide on the
activities of the hexokinase, phosphofructokinase and pyruvate kinase
were examined. Pieces of skeletal muscle (quadriceps) and liver were
obtained from 10 non-diabetic subjects during surgery. Tissues were
homogenized and the activities of the enzymes were measured in the
presence or absence of furosemide (0-1.5 mM). Furosemide inhibited the
activity of all three key glycolytic enzymes. The concentration of
furosemide required to inhibit phosphofructokinase in muscle was lower
than that required to inhibit the activity of this enzyme in the liver
or to inhibit the activities of hexokinase and pyruvate kinase in both
muscle and liver. These direct effects of furosemide may contribute to
the decrease in glucose utilisation following therapy with this and
similar agents in man.
ER -