TY - JOUR
TI - THE RAT PROBASIN GENE PROMOTER DIRECTS HORMONALLY AND
DEVELOPMENTALLY-REGULATED EXPRESSION OF A HETEROLOGOUS GENE SPECIFICALLY
TO THE PROSTATE IN TRANSGENIC MICE
AU - GREENBERG, NM
AU - DEMAYO, FJ
AU - SHEPPARD, PC
AU - BARRIOS, R and
AU - LEBOVITZ, R
AU - FINEGOLD, M
AU - ANGELOPOULOU, R
AU - DODD, JG and
AU - DUCKWORTH, ML
AU - ROSEN, JM
AU - MATUSIK, RJ
JO - MOLECULAR ENDOCRINOLOGY
PY - 1994
VL - 8
TODO - 2
SP - 230-239
PB - ENDOCRINE SOC
SN - 0888-8809
TODO - 10.1210/me.8.2.230
TODO - null
TODO - An expression cassette carrying 426 basepairs of the rat probasin (PB)
gene promoter and 28 basepairs of 5’-untranslated region is sufficient
to target the expression of the bacterial chloramphenicol
acetyltransferase (CAT) gene specifically to the prostate in transgenic
mice. The PB-CAT transgene was expressed in three of five (60%)
independent lines of mice, and this expression, as reported previously
for the endogenous rat gene, was male specific, restricted primarily to
the lateral, dorsal, and ventral lobes of the prostate, with only very
low levels of CAT activity detected in the anterior prostate and seminal
vesicles. The developmental and hormonal regulation of the transgene
also paralleled that reported for the rat gene, with a 70-fold increase
in CAT activity in the mouse prostate observed between 2-7 weeks of age,
a time corresponding to sexual maturation. PB-CAT activity in the
prostate declined after castration to 3.5% of the precastration level,
and the CAT activity in castrated males approached precastration levels
when mice were supplemented with testosterone. Transgene expression in
castrated males was not induced by dexamethasone. Coinjection of PB-CAT
with a chicken lysozyme gene matrix attachment region resulted in their
cointegration and further restricted the pattern of PB-CAT to the
dorsolateral prostate, with suppressed expression observed in the
ventral prostate. These studies demonstrate that a minimal rat probasin
promoter can target heterologous gene expression specifically to the
prostate in a developmentally and hormonally regulated fashion.
ER -