TY - JOUR
TI - Herpesvirus-like DNA sequences detected in endemic, classic, iatrogenic
and epidemic Kaposi's sarcoma (KS) biopsies
AU - Buonaguro, FM
AU - Tornesello, ML
AU - BethGiraldo, E
AU - Hatzakis, A and
AU - Mueller, N
AU - Downing, R
AU - Biryamwaho, B
AU - Sempala, SDK and
AU - Giraldo, G
JO - International Journal  of Cancer
PY - 1996
VL - 65
TODO - 1
SP - 25-28
PB - Wiley
SN - 0020-7136
TODO - 10.1002/(SICI)1097-0215(19960103)65:1<25::AID-IJC5>3.0.CO;2-3
TODO - null
TODO - The identification of Kaposi’s sarcoma (KS) clusters in sub-equatorial
Africa (endemic KS, AKS) and the high frequency of KS in sexually
transmitted AIDS (epidemic KS, EKS), have previously suggested a role
for infectious agents in the etiopathogenesis of KS. The recent
identification of herpesvirus (HHV)-like DNA sequences in one Ease of
EKS and their detection in >90% of all tested EKS, prompted us to
determine the prevalence of these viral sequences in all types of KS,
such as AKS, EKS, classic KS (CKS) and iatrogenic KS (IKS). The presence
of herpesvirus(HHV)-like DNA sequences has been examined in 61 KS skin
tumors obtained from Greece, Italy, USA, Uganda and Kenya. All KS
types(100%) were positive by polymerase chain reaction (PCR) and
Southern-blot analysis, while 5 out of b (83%) and 4 out of 7 (57%)
uninvolved autologous skin biopsies from AKS and CKS patients,
respectively, were positive for HHV-like sequences. All samples from
non-KS patients were negative, i.e. 17 human biopsies from healthy
individuals or patients affected by other pathologies, 5 human cell
lines and 15 peripheral blood mononuclear cells (PBMC) from HIV-positive
subjects. These results suggest that HHV-like sequences play a major
role in the pathogenesis of this neoplasm. (C) 1996 Wiley-Liss, Inc.
ER -