TY - JOUR
TI - Localization of the glucocorticoid receptor in rat brain mitochondria
AU - Moutsatsou, P
AU - Psarra, AMG
AU - Tsiapara, A
AU - Paraskevakou, H and
AU - Davaris, P
AU - Sekeris, CE
JO - Archives of Biochemistry and Biophysics
PY - 2001
VL - 386
TODO - 1
SP - 69-78
PB - Academic Press Inc.
SN - 0003-9861, 1096-0384
TODO - 10.1006/abbi.2000.2162
TODO - synaptosomes; hormone action; hippocampus; cerebral cortex;
mitochondria; glucocorticoid receptor
TODO - The distribution of glucocorticoid receptor in subcellular fractions of
brain cortex and hippocampus, two regions rich in glucocorticoid
receptor, has revealed its presence in nuclei, cytosol, mitochondria,
synaptosomes, and synaptosomal mitochondria, The identification of
glucocorticoid receptor has been accomplished both by Western blotting
using antibodies recognizing the carboxy and the amino terminus of the
glucocorticoid receptor and by immunogold electron microscopy using the
same anti-glucocorticoid receptor antibodies. Antibody-glucocorticoid
receptor interaction is abolished by preincubation of each antibody with
its competing peptide. In addition to the intact 95-kDa glucocorticoid
receptor in all fractions, lower molecular weight glucocorticoid
receptor fragments have been also detected by Western blotting. The
presence of glucocorticoid receptor in brain mitochondria supports the
concept of a direct action of glucocorticoids on mitochondrial gene
transcription, parallel to the established primary actions of the
hormones on nuclear gene transcription, as a mechanism of coordinate
regulation of respiratory enzyme biosynthesis by steroid hormones. (C)
2001 Academic Press.
ER -