TY - JOUR
TI - Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis
AU - Miliaraki, M.
AU - Briassoulis, P.
AU - Ilia, S.
AU - Polonifi, A.
AU - Mantzourani, M.
AU - Briassouli, E.
AU - Vardas, K.
AU - Nanas, S.
AU - Pistiki, A.
AU - Theodorakopoulou, M.
AU - Tavladaki, T.
AU - Spanaki, A.M.
AU - Kondili, E.
AU - Dimitriou, H.
AU - Tsiodras, S.
AU - Georgopoulos, D.
AU - Armaganidis, A.
AU - Daikos, G.
AU - Briassoulis, G.
JO - Scientific Reports
PY - 2021
VL - 11
TODO - 1
SP - null
PB - Institute of Geographic Sciences and Natural Resources Research
SN - 2045-2322
TODO - 10.1038/s41598-020-78208-2
TODO - baculoviral IAP repeat containing protein 5;  BIRC5 protein, human;  caspase;  caspase 3;  caspase 9;  messenger RNA, blood;  case control study;  clinical trial;  female;  human;  male;  metabolism;  middle aged;  mortality;  multicenter study;  prospective study;  real time polymerase chain reaction;  sepsis;  systemic inflammatory response syndrome, Case-Control Studies;  Caspase 3;  Caspase 9;  Caspases;  Female;  Humans;  Male;  Middle Aged;  Prospective Studies;  Real-Time Polymerase Chain Reaction;  RNA, Messenger;  Sepsis;  Survivin;  Systemic Inflammatory Response Syndrome
TODO - Sepsis is a dysregulated host response to infection related to devastating outcomes. Recently, interest has been shifted towards apoptotic and antiapoptotic pathobiology. Apoptosis is executed through the activation of caspases regulated by a number of antiapoptotic proteins, such as survivin. The survivin and caspases’ responses to sepsis have not yet been elucidated. This is a multicenter prospective observational study concerning patients with sepsis (n = 107) compared to patients with traumatic systemic inflammatory response syndrome (SIRS) (n = 75) and to healthy controls (n = 89). The expression of survivin was quantified through real-time quantitative polymerase chain reaction for the different survivin splice variants (wild type-WT, ΔEx3, 2B, 3B) in peripheral blood leukocytes. The apoptotic or antiapoptotic tendency was specified by measuring survivin-WT, caspase-3, and -9 serum protein concentrations through enzyme-linked immunosorbent assay. The survivin-WT, -2B, -ΔΕx3 mRNA, survivin protein, and caspases showed an escalated increase in SIRS and sepsis, whereas survivin-3B was repressed in sepsis (p < 0.05). Survivin correlated with IL-8 and caspase-9 (p < 0.01). For discriminating sepsis, caspase-9 achieved the best receiver operating characteristic curve (AUROC) of 0.95. In predicting mortality, caspase-9 and survivin protein achieved an AUROC of 0.70. In conclusion, specific apoptotic and antiapoptotic pathways might represent attractive targets for future research in sepsis. © 2021, The Author(s).
ER -