TY - JOUR
TI - Detection of herplex simplex virus-1 and -2 in cardiac myxomas
AU - Pateras, I.S.
AU - Evangelou, K.
AU - Tsimaratou, K.
AU - Liontos, M.
AU - Sakellariou, S.
AU - Barlogiannis, T.
AU - Karakitsos, P.
AU - Papalois, A.
AU - Kotsinas, A.
AU - Gorgoulis, V.G.
JO - Journal of Biomedicine and Biotechnology
PY - 2012
VL - 2012
TODO - null
SP - null
PB - 
SN - 1110-7243, 1110-7251
TODO - 10.1155/2012/823949
TODO - virus DNA;  virus DNA, article;  Chinese;  clinical article;  controlled study;  gene sequence;  heart;  heart myxoma;  herpes simplex;  Herpes simplex virus 1;  Herpes simplex virus 2;  human;  human tissue;  microscopy;  nonhuman;  nucleotide sequence;  polymerase chain reaction;  restriction fragment length polymorphism;  virus detection;  aged;  case control study;  chemistry;  cytochemistry;  female;  genetics;  heart atrium;  heart tumor;  heart ventricle;  herpes simplex;  isolation and purification;  male;  middle aged;  myxoma;  nonparametric test;  pathology;  virology, Human herpesvirus 1;  Simplexvirus, Aged;  Case-Control Studies;  DNA, Viral;  Female;  Heart Atria;  Heart Neoplasms;  Heart Ventricles;  Herpes Simplex;  Herpesvirus 1, Human;  Herpesvirus 2, Human;  Histocytochemistry;  Humans;  Male;  Middle Aged;  Myxoma;  Polymerase Chain Reaction;  Polymorphism, Restriction Fragment Length;  Statistics, Nonparametric
TODO - The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences. Copyright 2012 Ioannis S. Pateras et al.
ER -