TY - JOUR
TI - Effects of Sideritis euboea (lamiaceae) aqueous extract on IL-6, OPG and RANKL secretion by osteoblasts
AU - Kassi, E.
AU - Paliogianni, A.
AU - Dontas, I.
AU - Aligiannis, N.
AU - Halabalaki, M.
AU - Papoutsi, Z.
AU - Skaltsounis, A.-L.
AU - Moutsatsou, P.
JO - Natural Product Communications
PY - 2011
VL - 6
TODO - 11
SP - 1689-1696
PB - Natural Product Incorporation
SN - null
TODO - 10.1177/1934578x1100601131
TODO - interleukin 6;  osteoclast differentiation factor;  osteoprotegerin;  Sideritis euboea extract;  Sideritis extract;  unclassified drug, animal cell;  article;  cell line;  concentration response;  controlled study;  drug effect;  drug isolation;  electrospray mass spectrometry;  high performance liquid chromatography;  in vitro study;  nonhuman;  osteoblast;  protein secretion;  Sideritis;  Sideritis euboea
TODO - The water extract obtained from the aerial parts of Sideritis euboea (Lamiaceae), which is known in Greece as 'mountain tea', was investigated by determining the in vitro effect of this extract on the IL-6, OPG and RANKL secretion by osteoblasts, three important molecules in osteoblast-osteoclast interplay. The results showed that this extract reduced significantly the secretion of IL-6 by KS-483 osteoblasts, while it also suppressed RANKL secretion, with both effects being dose-dependent and more potent at the higher concentrations tested (50, 100 μg/mL). We further determined the chemical profile of the extract by applying an analytical U-HPLC-DAD-ESI-MS/MS method using the high resolution hybrid LTQ-Orbitrap Discovery spectrometer. An ESI source in negative mode was employed. The analysis indicated that the water extract of S. euboea is rich in flavonoid glycosides, and phenylpropanoid glycosides.
ER -