TY - JOUR
TI - Uptake of BSA-FITC loaded PLGA nanoparticles by bone marrow-derived dendritic cells induces maturation but not IL-12 or IL-10 production
AU - Karagouni, E.
AU - Kammona, O.
AU - Margaroni, M.
AU - Kotti, K.
AU - Karageorgiou, V.
AU - Gaitanaki, C.
AU - Kiparissides, C.
JO - Nanoscience and Nanotechnology Letters
PY - 2013
VL - 5
TODO - 4
SP - 498-504
PB - 
SN - 1941-4900, 1941-4919
TODO - 10.1166/nnl.2013.1564
TODO - BSA-FITC;  DCs maturation;  IL-10;  IL-12;  PLGA nanoparticles, Bone;  Cells;  Copolymers;  Cytology;  Nanoparticles, Synthesis (chemical)
TODO - Nanoparticles prepared from biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), represent a new approach for vaccine delivery due to their ability to be taken up by phagocytes and activate immune responses. In this study, fluorescently labelled bovine serum albumin (BSA-FITC)-loaded PLGA nanoparticles, of an average size ∼300 nm were prepared and examined for their ability to be taken up by bone marrow-derived dendritic cells (BM-DCs) in vitro and thus to promote their maturation and activation. The synthesized nanoparticles did not exhibit any cytotoxic or hemolytic effect and were taken up by BM-DCs efficiently, in a time and dose dependent manner. The localization of BSA-FITC loaded PLGA nanoparticles both in the acidophilic cellular compartments and the cytoplasm resulted in the maturation of BM-DCs expressing higher levels of costimulatory and MHC class II molecules in comparison to empty PLGA nanoparticles. However, the absence of IL-12 or IL-10 production indicates partial activation of BM-DCs suggesting the necessity of an adjuvant addition in order to facilitate DCs functionalization. Copyright © 2013 American Scientific Publishers. All rights reserved.
ER -