TY - JOUR
TI - Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method
AU - Espinel-Ingroff, A.
AU - Alvarez-Fernandez, M.
AU - Cantón, E.
AU - Carver, P.L.
AU - Chen, S.C.-A.
AU - Eschenauer, G.
AU - Getsinger, D.L.
AU - Gonzalez, G.M.
AU - Govender, N.P.
AU - Grancini, A.
AU - Hanson, K.E.
AU - Kidd, S.E.
AU - Klinker, K.
AU - Kubin, C.J.
AU - Kus, J.V.
AU - Lockhart, S.R.
AU - Meletiadis, J.
AU - Morris, A.J.
AU - Pelaez, T.
AU - Quindós, G.
AU - Rodriguez-Iglesias, M.
AU - Sánchez-Reus, F.
AU - Shoham, S.
AU - Wengenack, N.L.
AU - Borrell Solé, N.
AU - Echeverria, J.
AU - Esperalba, J.
AU - De La Pedrosa, E.G.-G.
AU - García García, I.
AU - Linares, M.J.
AU - Marco, F.
AU - Merino, P.
AU - Pemán, J.
AU - Pérez Del Molino, L.
AU - Roselló Mayans, E.
AU - Rubio Calvo, C.
AU - Ruiz Pérez De Pipaon, M.
AU - Yagüe, G.
AU - Garcia-Effron, G.
AU - Guinea, J.
AU - Perlin, D.S.
AU - Sanguinetti, M.
AU - Shields, R.
AU - Turnidge, J.
JO - Antimicrobial Agents and Chemotherapy
PY - 2015
VL - 59
TODO - 11
SP - 6725-6732
PB - American Society for Microbiology
SN - 0066-4804, 1098-6596
TODO - 10.1128/AAC.01250-15
TODO - anidulafungin;  caspofungin;  micafungin;  anidulafungin;  antifungal agent;  caspofungin;  echinocandin;  lipopeptide;  micafungin, antifungal resistance;  antifungal susceptibility;  Article;  Australia;  Canada;  Candida;  Candida albicans;  Candida dubliniensis;  Candida glabrata;  Candida parapsilosis;  Candida tropicalis;  Clavispora lusitaniae;  colorimetry;  Europe;  fungal detection;  Mexico;  Meyerozyma guilliermondii;  minimum inhibitory concentration;  mutation;  New Zealand;  nonhuman;  Pichia kudriavzevii;  priority journal;  South Africa;  United States;  Candida;  drug effects;  genetics;  microbial sensitivity test, Antifungal Agents;  Candida;  Echinocandins;  Lipopeptides;  Microbial Sensitivity Tests;  Mutation
TODO - Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >97.5% of the statistically modeled population were, respectively, 0.12,0.25, and 0.06 μg/ml for C. albicans, 0.12, 0.25, and 0.03 μg/ml for C. glabrata complex, 4, 2, and 4 μg/ml for C. parapsilosis complex, 0.5,0.25, and 0.06 μg/ml for C. tropicalis, 0.25,1, and 0.25 μg/ml for C. krusei, 0.25,1, and 0.12 μg/ml for C. lusitaniae, 4,2, and 2 μg/ml for C. guilliermondii, and 0.25,0.25, and 0.12 μg/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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