TY - JOUR
TI - Phylogenetic lineages, clones and β-lactamases in an international collection of Klebsiella oxytoca isolates non-susceptible to expanded-spectrum cephalosporins
AU - Izdebski, R.
AU - Fiett, J.
AU - Urbanowicz, P.
AU - Baraniak, A.
AU - Derde, L.P.G.
AU - Bonten, M.J.M.
AU - Carmeli, Y.
AU - Goossens, H.
AU - Hryniewicz, W.
AU - Brun-Buisson, C.
AU - Brisse, S.
AU - Gniadkowski, M.
AU - Herda, M.
AU - Dautzenberg, M.J.
AU - Adler, A.
AU - Kazma, M.
AU - Navon-Venezia, S.
AU - Malhotra-Kumar, S.
AU - Lammens, C.
AU - Legrand, P.
AU - Chalfine, A.
AU - Giamarellou, H.
AU - Petrikkos, G.L.
AU - Balode, A.
AU - Dumpis, U.
AU - Stammet, P.
AU - Aragăo, I.
AU - Esteves, F.
AU - Torres Martí, A.
AU - Lawrence, C.
AU - Salomon, J.
AU - Paul, M.
AU - Lerman, Y.
AU - Rossini, A.
AU - Salvia, A.
AU - Vidal Samso, J.
AU - Fierro, J.
AU - MOSAR WP2, WP3
AU - WP5 Study Groups
JO - The Journal of antimicrobial chemotherapy
PY - 2015
VL - 70
TODO - 12
SP - 3230-3237
PB - Oxford University Press
SN - null
TODO - 10.1093/jac/dkv273
TODO - bacterial enzyme;  beta lactamase AmpC;  carbapenemase;  cephalosporin derivative;  cephalosporinase;  extended spectrum beta lactamase;  OXY 1 beta lactamase;  OXY 2 beta lactamase;  OXY 3 beta lactamase;  OXY 4 beta lactamase;  OXY 5 beta lactamase;  unclassified drug;  antiinfective agent;  beta lactamase;  cephalosporin derivative, antibiotic resistance;  Article;  bacterial chromosome;  bacterial gene;  bacterial strain;  bacterium isolate;  clonal evolution;  comparative study;  controlled study;  enzyme analysis;  gene amplification;  gene sequence;  genetic variability;  genotype;  human;  hydrolysis;  Klebsiella oxytoca;  major clinical study;  nonhuman;  nucleotide sequence;  phenotype;  phylogenetic tree;  species diversity;  antibiotic resistance;  classification;  DNA sequence;  drug effects;  Europe;  feces;  genetic variation;  genetics;  genotype;  heterozygote;  hospital;  isolation and purification;  Israel;  Klebsiella Infections;  Klebsiella oxytoca;  microbial sensitivity test;  microbiology;  molecular typing;  phylogeny;  polymerase chain reaction, Anti-Bacterial Agents;  beta-Lactam Resistance;  beta-Lactamases;  Carrier State;  Cephalosporins;  Europe;  Feces;  Genetic Variation;  Genotype;  Hospitals;  Humans;  Israel;  Klebsiella Infections;  Klebsiella oxytoca;  Microbial Sensitivity Tests;  Molecular Typing;  Phylogeny;  Polymerase Chain Reaction;  Sequence Analysis, DNA
TODO - Objectives: The objective of this study was to examine Klebsiella oxytoca clonal and phylogenetic diversity, based on an international collection of carriage isolates non-susceptible to expanded-spectrum cephalosporins (ESCs). Methods: The study material comprised 68 rectal carriage K. oxytoca isolates non-susceptible to ESCs recovered in 2008-11 from patients in 14 hospitals across Europe and Israel. ESC resistance was tested phenotypically; genes encoding ESBLs, AmpC cephalosporinases and carbapenemases were amplified and sequenced. The isolates were typed by PFGE and MLST, followed by sequencing of blaOXY genes. Results: MLSTand PFGE distinguished 34 STs and 47 pulsotypes among the isolates, respectively. Six STswere split into several pulsotypes each. Five STs were more prevalent (n=2-9) and occurred in several countries each, including ST2, ST9 and ST141, which belong to a growing international clonal complex (CC), CC2. Four phylogenetic lineages were distinguished, each with another type of chromosomal OXY-type β-lactamase. Three of these, with OXY-1/-5, OXY-2 types and OXY-4, corresponded to previously described phylogroups KoI, KoII and KoIV, respectively. A single isolate from Israel represented a distinct lineage with a newly defined OXY-7 type. The phylogroups showed interesting differences in mechanisms of ESC resistance; KoI strains rarely overexpressed the OXY enzymes but commonly produced ESBLs, whereas KoII strains often were OXY hyperproducers and carried ESBLs much less frequently. AmpCs (DHA-1) and carbapenemases (VIM-1) occurred sporadically. Conclusions: The study confirmed the high genetic diversity of the collection of K. oxytoca ESC-non-susceptible isolates, composed of phylogroups with distinct types of OXY-type β-lactamases, and revealed some STs of broad geographical distribution.
ER -