TY - JOUR
TI - Effect of 7-O-β-D-glucopyranosylchrysin and its aglycone chrysin isolated from Podocytisus caramanicus on estrogen receptor α transcriptional activity
AU - Berthier, A.
AU - Girard, C.
AU - Grandvuillemin, A.
AU - Muyard, F.
AU - Skaltsounis, A.-L.
AU - Jouvenot, M.
AU - Delage-Mourroux, R.
JO - Planta Medica
PY - 2007
VL - 73
TODO - 14
SP - 1447-1451
PB - 
SN - 0032-0943, 1439-0221
TODO - 10.1055/s-2007-990248
TODO - 7 o beta glucopyranosylchrysin;  aglycone chrysin;  chrysin;  estrogen receptor alpha;  fulvestrant;  plant extract;  Podocytisus caramanicus extract;  unclassified drug, antineoplastic activity;  article;  cell strain MCF 7;  drug efficacy;  estrogen activity;  female;  genetic transcription;  genetic transfection;  human;  human cell;  legume;  medicinal plant;  Podocytisus caramanicus, Antineoplastic Agents, Phytogenic;  Breast Neoplasms;  Cell Line, Tumor;  Estrogen Receptor alpha;  Fabaceae;  Flavonoids;  Glucosides;  Humans;  Molecular Structure;  Transcription, Genetic, Fabaceae
TODO - A screening of Greek Fabaceae extracts identified the methanolic extract of Podocytisus caramanicus Boiss. & Heldr. as having proliferative activity on human breast cancer cells (MCF-7). Using transient transfection experiments, we have first used three compounds described for their estrogen-like properties, E2, genistein (Gen) and biochanin A (Bch), as controls to evaluate our cellular model. Secondly, we have demonstrated that the 7-O-β-D- glucopyranosylchrysin (Glc-chr), the most abundant flavone of the extract, and its aglycone chrysin were able to increase estrogen receptor α transcriptional activity in MCF-7 cells. We have also shown that the estrogenic activity of Glc-chr could be completely suppressed by the pure estrogen antagonist ICI 182,780 suggesting that the effect of Glc-chr is mediated by ERα. © Georg Thieme Verlag KG Stuttgart.
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