TY - JOUR
TI - Evaluation of the anti-inflammatory and cytotoxic activities of naphthazarine derivatives from Onosma leptantha
AU - Kundakovic, T.
AU - Fokialakis, N.
AU - Dobric, S.
AU - Pratsinis, H.
AU - Kletsas, D.
AU - Kovacevic, N.
AU - Chinou, I.
JO - Phytomedicine
PY - 2006
VL - 13
TODO - 4
SP - 290-294
PB - Urban und Fischer Verlag Jena
SN - 0944-7113
TODO - 10.1016/j.phymed.2004.10.009
TODO - acetylshikonin;  antiinflammatory agent;  beta,beta dimethylacrylshikonin;  carrageenan;  indometacin;  isovalerylshikonin;  naphthazarine derivative;  Onosma leptantha extract;  plant extract;  shikonin derivative;  unclassified drug, animal cell;  animal experiment;  animal model;  antiinflammatory activity;  article;  bioassay;  cell line;  controlled study;  cytotoxicity;  fibrosarcoma;  fractionation;  herbal medicine;  human;  human cell;  lymphatic leukemia;  male;  nonhuman;  Onosma leptantha;  paw edema;  plant root;  priority journal;  rat, Animalia;  Murinae;  Onosma
TODO - The root extracts of Onosma leptanhtha were evaluated for their anti-iflammatory and cytotoxic activities. The cyclohexane extract, which appeared as the most active in both assays, has been further subjected to bioassay-directed fractionation to afford the naphthazarine derivatives: β,β-dimethylacrylshikonin (1), isovalerylshikonin (2) and acetylshikonin (3). The evaluation of the anti-inflammatory activity was performed on carrageenan-induced rat paw edema test. All the tested compounds proved to be active, while compound 3 showed the best anti-inflammatory effect. In addition, the cytotoxic activity of the extracts and isolated compounds, was also assayed against L1210 murine lymphoblastic leukemia cell line, and human fibrosarcoma HT-1080 cells. Compound 1 exhibited remarkable cytotoxic activity (390 nM for L1210 cells), which is superior to that of shikonin, which was used as control. © 2005 Elsevier GmbH. All rights reserved.
ER -