TY - JOUR
TI - Biological effect of silver-modified nanostructured titanium dioxide in cancer
AU - Lagopati, N.
AU - Kotsinas, A.
AU - Veroutis, D.
AU - Evangelou, K.
AU - Papaspyropoulos, A.
AU - Arfanis, M.
AU - Falaras, P.
AU - Kitsiou, P.V.
AU - Pateras, I.
AU - Bergonzini, A.
AU - Frisan, T.
AU - Kyriazis, S.
AU - Tsoukleris, D.S.
AU - Tsilibary, E.-P.C.
AU - Gazouli, M.
AU - Pavlatou, E.A.
AU - Gorgoulis, V.G.
JO - Cancer Genomics and Proteomics
PY - 2021
VL - 18
TODO - null
SP - 425-439
PB - International Institute of Anticancer Research
SN - null
TODO - 10.21873/CGP.20269
TODO - silver nanoparticle;  titanium dioxide nanoparticle;  nanomaterial;  silver;  titanium;  titanium dioxide, antineoplastic activity;  apoptosis;  Article;  biological activity;  cell culture;  cell viability;  colorimetry;  cytotoxicity;  DNA fragmentation assay;  human;  human cell;  MCF-7 cell line;  MDA-MB-231 cell line;  MTT assay;  photoactivation;  protein expression;  sol-gel;  Western blotting;  chemistry;  nanomedicine;  neoplasm;  procedures, Humans;  Nanomedicine;  Nanostructures;  Neoplasms;  Silver;  Titanium
TODO - Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions. © 2021 International Institute of Anticancer Research. All rights reserved.
ER -