TY - JOUR
TI - Adiponectin and Its Effects on Acute Leukemia Cells: An Experimental and Bioinformatics Approach
AU - Tsartsalis, A.N.
AU - Tagka, A.
AU - Kotoulas, A.
AU - Mirkopoulou, D.
AU - Geronikolou, S.A.
AU - G, L.
JO - Advances in Experimental Medicine and Biology
PY - 2021
VL - 1338
TODO - null
SP - 117-127
PB - Springer-Verlag
SN - null
TODO - 10.1007/978-3-030-78775-2_14
TODO - adiponectin;  membrane protein;  SPINT2 protein, human, acute lymphoblastic leukemia;  biology;  genetics;  human;  oncogene;  tumor cell line, Adiponectin;  Cell Line, Tumor;  Computational Biology;  Humans;  Membrane Glycoproteins;  Oncogenes;  Precursor Cell Lymphoblastic Leukemia-Lymphoma
TODO - Acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy. It is known that deregulation of adipokine pathways is probably implicated in the ontogenesis of ALL. The present work aims at investigating the role of adiponectin and its effects on an ALL cell line. The CCRF-CEM cells were used as a model. Cells have been treated with adiponectin, with different concentrations up to 72 h. Cytotoxicity and cell cycle distribution were investigated for all concentrations using flow cytometry. Selected concentrations were also used for additional microarray analysis, using a small gene set of cancer-related genes. Lower and higher adiponectin concentrations did not produce an inhibition of proliferation, as well as an increase in cell death. It was found that adiponectin regulated differentially genes, such as CD22, CDH1, IFNG, LCK, MSH2, SPINT2, and others. At the same time, it appeared that adiponectin-related gene expression was more active on chromosomes 18 and 1. Machine learning classification algorithms showed that several genes were grouped together indicating common regulatory mechanisms. The present study showed that adiponectin is able to induce gene differential expression in leukemic cells in vitro, suggesting a possible role in the progression of leukemia. It is also an indication that more studies are required in order to further understand the role of adiponectin and adipokines in general in the role of human neoplasms. © 2021, The Author(s), under exclusive license to Springer Nature Switzerland AG.
ER -