TY - JOUR
TI - MicroRNA-92a-3p overexpression in peripheral blood mononuclear cells is an independent predictor of prolonged overall survival of patients with chronic lymphocytic leukemia
AU - Papageorgiou, S.G.
AU - Diamantopoulos, M.A.
AU - Kontos, C.K.
AU - Bouchla, A.
AU - Vasilatou, D.
AU - Bazani, E.
AU - Scorilas, A.
AU - Pappa, V.
JO - Clinical Lymphoma Myeloma and Leukemia
PY - 2019
VL - 60
TODO - 3
SP - 658-667
PB - Taylor and Francis Ltd.
SN - null
TODO - 10.1080/10428194.2018.1461861
TODO - lactate dehydrogenase;  microRNA;  microRNA 92a 3p;  unclassified drug;  immunoglobulin heavy chain;  microRNA;  MIRN92 microRNA, human;  tumor marker, adult;  aged;  amplicon;  Article;  blood donor;  cancer prognosis;  cancer survival;  chronic lymphatic leukemia;  cohort analysis;  female;  flow cytometry;  follow up;  housekeeping gene;  human;  human cell;  immunophenotyping;  in vitro study;  intermediate risk patient;  lactate dehydrogenase blood level;  major clinical study;  male;  mRNA expression level;  overall survival;  peripheral blood mononuclear cell;  polyadenylation;  priority journal;  protein expression;  real time polymerase chain reaction;  reverse transcription;  Sanger sequencing;  signal transduction;  U-937 cell line;  very elderly;  case control study;  chronic lymphatic leukemia;  gene expression regulation;  genetics;  Kaplan Meier method;  metabolism;  middle aged;  mononuclear cell;  mortality;  mutation;  prognosis;  proportional hazards model;  tumor cell line, Aged;  Aged, 80 and over;  Biomarkers, Tumor;  Case-Control Studies;  Cell Line, Tumor;  Female;  Gene Expression Regulation, Neoplastic;  Humans;  Immunoglobulin Heavy Chains;  Kaplan-Meier Estimate;  Leukemia, Lymphocytic, Chronic, B-Cell;  Leukocytes, Mononuclear;  Male;  MicroRNAs;  Middle Aged;  Mutation;  Prognosis;  Proportional Hazards Models
TODO - MicroRNA-92a-3p (miR-92a-3p) derives from the oncogenic miR-17/92 cluster and its highly homologous miR-106a/363 cluster. miR-92a-3p regulates the expression of key transcription factors such as HIF1 and inhibits SOCS1 to enhance the anti-apoptotic STAT3/IL6 signaling pathway. In this study, we assessed the putative usefulness of miR-92a-3p as a prognostic and/or diagnostic biomarker in chronic lymphocytic leukemia (CLL). For this purpose, total RNA was extracted from mononuclear cells isolated from the peripheral blood of 88 CLL patients and 36 non-leukemic blood donors, was polyadenylated and reversely transcribed. miR-92a-3p expression was quantified using an accurate qPCR method. miR-92a-3p levels were significantly lower in peripheral blood mononuclear cells of CLL patients. Overall survival (OS) analysis revealed that high miR-92a-3p expression predicts significantly prolonged OS of CLL patients. Interestingly, miR-92a-3p overexpression remains a significant prognosticator in subgroups of CLL patients with distinct prognosis. In conclusion, miR-92a-3p overexpression is a potential surrogate biomarker of favorable outcome of CLL patients. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
ER -