TY - JOUR
TI - Mutation detection of the human glucocorticoid receptor alpha gene area
coding for the hormone-binding domain by denaturing gradient gel
electrophoresis
AU - Tsolakidou, AF
AU - Coulocheri, SA
AU - Sekeris, CE
AU - Moutsatsou, P
JO - Journal of Biochemical and Biophysical Methods
PY - 2002
VL - 51
TODO - 3
SP - 233-241
PB - ELSEVIER SCIENCE BV
SN - 0165-022X
TODO - 10.1016/S0165-022X(02)00012-X
TODO - DGGE; glucocorticoid receptor; mutation detection
TODO - Mutations in the hormone-binding domain of the human glucocorticoid
receptor alpha (hGRalpha) gene have been detected in a variety of
glucocorticoid resistance syndromes. Using the denaturing gradient gel
electrophoresis technique, we developed a sensitive method for the
detection of alterations in the gene area coding for the whole
hormone-binding domain and part of the DNA-binding domain of the
hGRalpha. This method can be applied for screening of glucocorticoid
receptor gene alterations in glucocorticoid-dependent diseases. (C) 2002
Elsevier Science B.V. All tights reserved.
ER -