TY - JOUR
TI - Detection of genetic abnormalities in neoplasms from Greek patients with
FAP
AU - Michalopoulos, NV
AU - Saetta, A
AU - Lazaris, AC
AU - Voutsinas, G and
AU - Davaris, PS
JO - European Journal of Surgical Oncology
PY - 2003
VL - 29
TODO - 1
SP - 38-43
PB - W B SAUNDERS CO LTD
SN - 0748-7983
TODO - 10.1053/ejso.2002.1344
TODO - FAP; microsatellite instability; p53; K-ras
TODO - Aims: DNA microsatellite instability is a well-known feature of
hereditary non-polyposis colon cancer; however, its incidence in
familial adenomatous polyposis, is unclear. We report the frequency of
microsatellite instability and other genetic abnormalities in a group of
Greek patients with FAP, in relation to various clinicopathological
variables.
Methods: Thirty-four tissue specimens from 10 patients with FAP were
studied. Microsatellite instability was investigated at six loci: BAT25,
BAT26, D2S123, D5S346, D17S250 and TGF-beta R11 poly(A) tract. p53 and
K-ras mutations were also examined.
Results: Microsatellite instability was detected in two FAP
adenocarcinomas from different patients. Mutation percentages observed
were: in K-ras 45% and 50% and in p53 14% and 58%, of FAP adenomas
and adenocarcinomas, respectively. No K-ras or p53 mutations were
determined in the two microsatellite instable adenocarcinomas.
Conclusion: Microsatellite instability is detectable in a small
proportion of adenocarcinomas complicating FAP. This minority of cases
may constitute a distinct subgroup among FAP neoplasms. (C) 2002,
Elsevier Science Ltd. All rights reserved.
ER -