TY - JOUR
TI - Proteomic analysis of normal and cancer cervical cell lines reveals deregulation of cytoskeleton-associated proteins
AU - Pappá, K.I.
AU - Lygirou, V.
AU - Kontostathi, G.
AU - Zoidakis, J.
AU - Makridakis, M.
AU - Vougas, K.
AU - Daskalakis, G.
AU - Polyzos, A.
AU - Anagnou, N.P.
JO - Cancer Genomics and Proteomics
PY - 2017
VL - 14
TODO - 4
SP - 253-266
PB - International Institute of Anticancer Research
SN - null
TODO - 10.21873/cgp.20036
TODO - actin;  actin depolymerizing factor;  cell extract;  cofilin 1;  cytoskeleton protein;  cytoskeleton protein, actin filament;  Article;  bioinformatics;  carcinogenesis;  cervical cancer cell line;  controlled study;  female;  HeLa cell line;  human;  human cell;  Human papillomavirus type 16;  Human papillomavirus type 18;  in vitro study;  keratinocyte;  matrix assisted laser desorption ionization time of flight mass spectrometry;  protein analysis;  protein expression;  protein function;  proteomics;  signal transduction;  two dimensional electrophoresis;  upregulation;  uterine cervix;  virus strain;  Wart virus;  Western blotting;  matrix-assisted laser desorption-ionization mass spectrometry;  metabolism;  pathology;  tumor cell line;  two dimensional gel electrophoresis;  uterine cervix tumor, Cell Line, Tumor;  Cytoskeletal Proteins;  Electrophoresis, Gel, Two-Dimensional;  Female;  Humans;  Proteomics;  Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;  Uterine Cervical Neoplasms
TODO - Background: Both HPV-positive and -negative cervical cancers are primarily associated with features of cell cycle and cytoskeletal disruption; however, the actual biological processes affected remain elusive. To this end, we systematically characterized the intracellular proteomic profiles of four distinct and informative cervical cell lines. Materials and Methods: Cell extracts from a normal cervical (HCK1T) and three cervical cancer cell lines, one HPVnegative (C33A), and two HPV-positive, SiHa (HPV16+) and HeLa (HPV18+), were analyzed by 2-dimensional electrophoresis and differentially expressed proteins were identified by MALDI-TOF mass spectrometry, while differential expression was confirmed by western blot analysis. Results: In total, 133 proteins were found differentially expressed between the normal and the cervical cancer lines. Bioinformatics analysis revealed the actin cytoskeleton signaling pathway to be significantly affected, while up-regulation of cofilin-1, an actin depolymerizing factor, was documented and further validated by western blotting. Furthermore, two-way comparisons among the four cell lines, revealed a set of 18 informative differentially expressed proteins. Conclusion: These novel identified proteins provide the impetus for further functional studies to dissect the mechanisms operating in the two distinct pathways of cervical carcinogenesis.
ER -