TY - JOUR
TI - Elevated miR-20b-5p expression in peripheral blood mononuclear cells: A novel, independent molecular biomarker of favorable prognosis in chronic lymphocytic leukemia
AU - Papageorgiou, S.G.
AU - Kontos, C.K.
AU - Tsiakanikas, P.
AU - Stavroulaki, G.
AU - Bouchla, A.
AU - Vasilatou, D.
AU - Bazani, E.
AU - Lazarakou, A.
AU - Scorilas, A.
AU - Pappa, V.
JO - Leukemia Research
PY - 2018
VL - 70
TODO - null
SP - 1-7
PB - Elsevier Ireland Ltd
SN - 0145-2126
TODO - 10.1016/j.leukres.2018.04.014
TODO - ADP ribosyl cyclase/cyclic ADP ribose hydrolase 1;  biological marker;  complementary DNA;  immunoglobulin heavy chain;  microRNA 20b;  microRNA 20b 5p;  RNA;  unclassified drug;  immunoglobulin heavy chain;  microRNA;  MIRN20b microRNA, human;  tumor marker, adult;  aged;  Article;  cancer prognosis;  chronic lymphatic leukemia;  controlled study;  female;  gene expression;  human;  human cell;  IGHV gene;  intermediate risk patient;  Kaplan Meier method;  major clinical study;  male;  mutational analysis;  overall survival;  peripheral blood mononuclear cell;  polyadenylation;  prediction;  priority journal;  promoter region;  proportional hazards model;  protein expression;  quantitative analysis;  real time polymerase chain reaction;  RNA isolation;  synthesis;  very elderly;  cancer staging;  chronic lymphatic leukemia;  gene expression regulation;  genetics;  metabolism;  middle aged;  mononuclear cell;  mortality;  mutation;  prognosis;  tumor cell line, Aged;  Biomarkers, Tumor;  Cell Line, Tumor;  Female;  Gene Expression Regulation, Leukemic;  Humans;  Immunoglobulin Heavy Chains;  Kaplan-Meier Estimate;  Leukemia, Lymphocytic, Chronic, B-Cell;  Leukocytes, Mononuclear;  Male;  MicroRNAs;  Middle Aged;  Mutation;  Neoplasm Staging;  Prognosis;  Proportional Hazards Models
TODO - MicroRNA-20b-5p (miR-20b-5p) is part of the miR-106a/363 cluster and a member of the cancer-related miR-17 family. miR-20b-5p regulates important transcription factors, including hypoxia-inducible factor 1 (HIF1) and signal transducer and activator of transcription 3 (STAT3). Recently, the dysregulation of miR-20b-5p expression has been observed in many B-cell lymphomas and T-cell leukemias. In this research study, we examined the putative prognostic value of miR-20b-5p in CLL. Therefore, total RNA was isolated from peripheral blood mononuclear cells (PBMCs) collected from 88 CLL patients; next, total RNA was polyadenylated and first-strand cDNA was synthesized, using an oligo-dT–adapter primer. miR-20b-5p expression was quantified using an in-house–developed real-time quantitative PCR assay. Kaplan-Meier OS analysis and bootstrap univariate Cox regression showed that high miR-20b-5p expression predicts better OS for CLL patients (p < 0.001). Interestingly, miR-20b-5p overexpression retains its favorable prognostic role in CLL patients of intermediate risk or stratified according to established prognostic factors [CD38 expression and mutational status of the immunoglobulin heavy chain variable (IGHV) region]. In conclusion, miR-20b-5p is a potential independent molecular biomarker of favorable prognosis in CLL. © 2018
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