TY - JOUR
TI - Potential sperm contributions to the murine zygote predicted by in silico analysis
AU - Ntostis, P.
AU - Carter, D.
AU - Iles, D.
AU - Huntriss, J.
AU - Tzetis, M.
AU - Miller, D.
JO - MOLECULAR HUMAN REPRODUCTION
PY - 2017
VL - 154
TODO - 6
SP - 777-788
PB - BioScientifica Ltd
SN - null
TODO - 10.1530/REP-17-0097
TODO - binding protein;  F box protein;  F box protein 2;  histone deacetylase 11;  microtubule associated protein;  microtubule associated protein 1A;  microtubule associated protein 5;  poly(rC)binding protein 4;  ubiquitin protein ligase E3;  unclassified drug;  zinc finger protein;  zinc finger protein 821;  messenger RNA, animal cell;  Article;  computer model;  controlled study;  female;  gene expression regulation;  genetic analysis;  male;  next generation sequencing;  nonhuman;  priority journal;  RNA processing;  RNA sequence;  sperm;  zygote;  animal;  biological model;  biology;  C57BL mouse;  computer simulation;  fertility;  gene expression profiling;  gene regulatory network;  genetic database;  genetic transcription;  genetics;  metabolism;  mouse;  physiology;  pregnancy;  RNA stability;  signal transduction;  spermatozoon;  zygote, Animals;  Computational Biology;  Computer Simulation;  Databases, Genetic;  Female;  Gene Expression Profiling;  Gene Expression Regulation, Developmental;  Gene Regulatory Networks;  Male;  Mice;  Mice, Inbred C57BL;  Models, Genetic;  Pregnancy;  RNA Processing, Post-Transcriptional;  RNA Stability;  RNA, Messenger;  RNA, Messenger, Stored;  Signal Transduction;  Sperm-Ovum Interactions;  Spermatozoa;  Transcription, Genetic;  Zygote
TODO - Paternal contributions to the zygote are thought to extend beyond delivery of the genome and paternal RNAs have been linked to epigenetic transgenerational inheritance in different species. In addition, sperm-egg fusion activates several downstream processes that contribute to zygote formation, including PLC zeta-mediated egg activation and maternal RNA clearance. Since a third of the preimplantation developmental period in the mouse occurs prior to the first cleavage stage, there is ample time for paternal RNAs or their encoded proteins potentially to interact and participate in early zygotic activities. To investigate this possibility, a bespoke next-generation RNA sequencing pipeline was employed for the first time to characterise and compare transcripts obtained from isolated murine sperm, MII eggs and pre-cleavage stage zygotes. Gene network analysis was then employed to identify potential interactions between paternally and maternally derived factors during the murine egg-to-zygote transition involving RNA clearance, protein clearance and post-transcriptional regulation of gene expression. Our in silico approach looked for factors in sperm, eggs and zygotes that could potentially interact co-operatively and synergistically during zygote formation. At least five sperm RNAs (Hdac11, Fbxo2, Map1lc3a, Pcbp4 and Zfp821) met these requirements for a paternal contribution, which with complementary maternal co-factors suggest a wider potential for extra-genomic paternal involvement in the developing zygote. © 2017 Society for Reproduction and Fertility.
ER -