TY - JOUR
TI - A novel modification of the AOM/DSS model for inducing intestinal adenomas in mice
AU - Angelou, A.
AU - Andreatos, N.
AU - Antoniou, E.
AU - Zacharioudaki, A.
AU - Theodoropoulos, G.
AU - Damaskos, C.
AU - Garmpis, N.
AU - Yuan, C.
AU - Xiao, W.
AU - Theocharis, S.
AU - Zografos, G.
AU - Papalois, A.
AU - Margonis, G.A.
JO - ANTICANCER RESEARCH
PY - 2018
VL - 38
TODO - 6
SP - 3467-3470
PB - International Institute of Anticancer Research
SN - 0250-1291
TODO - 10.21873/anticanres.12616
TODO - azoxymethane;  dextran sulfate;  azoxymethane;  dextran sulfate, adenocarcinoma;  animal experiment;  animal model;  animal tissue;  Article;  colorectal adenoma;  controlled study;  dextran sulfate sodium-induced colitis;  female;  hyperplasia;  loose feces;  mononuclear cell;  mouse;  neutrophil chemotaxis;  nonhuman;  priority journal;  adenoma;  animal;  C57BL mouse;  chemically induced;  colitis;  colon tumor;  disease model;  human;  intestine tumor;  pathology, Adenoma;  Animals;  Azoxymethane;  Colitis;  Colonic Neoplasms;  Dextran Sulfate;  Disease Models, Animal;  Female;  Humans;  Intestinal Neoplasms;  Mice, Inbred C57BL
TODO - Background/Aim: Our aim was to develop an animal model of the precancerous stages of colitis-associated carcinogenesis by modifying the established azoxymethane/ dextran sulfate sodium (AOM/DSS) protocol. Materials and Methods: Six mice were treated with varying cycles of DSS following AOM administration as above (group 1: three mice received three 5-day cycles of 3.0% DSS and group 2: three mice received three 7-day cycles of 2.5% DSS; every cycle was followed by a 2-week rest period) and were sacrificed on day 84 of the experiment. By contrast, three female C57BL6 mice (group 3) were treated with a single intraperitoneal dose (10 mg/kg of body weight) of AOM followed by three 5-day cycles of oral 2.5% DSS, with each cycle interrupted by a 2-week rest period. The mice of this group were sacrificed at 60 days. Results: In groups 1 and 2, cancer was noted in five out of the six mice. In group 3, adenomas with dysplastic lesions were noted in all of the mice, but none had developed adenocarcinoma. Conclusion: Our results suggest that the administration of three 5-day cycles of 2.5% DSS following an initial dose of AOM may successfully induce adenoma formation without the concurrent presence of carcinoma in female C57BL6 mice that are sacrificed on experimental day 60. In turn, this modification of the widely used AOM/DSS protocol may constitute a novel approach for investigating colitis-related colonic adenomas. © 2018 International Institute of Anticancer Research. All rights reserved.
ER -