TY - JOUR
TI - Oxidative stress in ageing and disease development studied by FT-IR spectroscopy
AU - Anastassopoulou, J.
AU - Kyriakidou, M.
AU - Kyriazis, S.
AU - Mavrogenis, A.F.
AU - Mamareli, V.
AU - Mamarelis, I.
AU - Petra, M.
AU - Malesiou, E.
AU - Kotoulas, C.
AU - Kolovou, P.
AU - Koutoulakis, E.
AU - Markouizou, A.
AU - Theophanides, T.
JO - Mechanisms of Ageing and Development
PY - 2018
VL - 172
TODO - null
SP - 107-114
PB - Elsevier Ireland Ltd
SN - 0047-6374
TODO - 10.1016/j.mad.2017.11.009
TODO - carbonyl derivative;  peroxide, adult;  aortic valve;  Article;  beta sheet;  bone biopsy;  bone cancer;  bone malformation;  child;  collagen fibril;  controlled study;  disease course;  disease exacerbation;  ex vivo study;  Fourier transform infrared spectroscopy;  human;  human tissue;  irradiation;  lipid degradation;  lipid peroxidation;  lipophilicity;  oxidative stress;  priority journal;  protein conformation;  radiation dose;  aging;  aortic valve stenosis;  bone tumor;  calcinosis;  female;  femoral head;  heterotopic ossification;  infrared spectroscopy;  male;  metabolism;  pathology;  preschool child, Adult;  Aging;  Aortic Valve;  Aortic Valve Stenosis;  Bone Neoplasms;  Calcinosis;  Child;  Child, Preschool;  Female;  Femur Head;  Humans;  Male;  Ossification, Heterotopic;  Oxidative Stress;  Spectroscopy, Fourier Transform Infrared
TODO - FT-IR spectroscopy was used to investigate the effect of oxidative stress and to approach the mechanism on cancer bone demineralization, aortic valve mineralization and heterotopic ossification on disease development. The FT-IR spectra obtained from paediatric, adult bone and ex vivo irradiated adult healthy bone with a dose of 20 Gy were compared with those of healthy bone. The increase of band intensity changes of vasCH2 vsCH2 in the region 3000–2850 cm−1 depended on aging, the disease progression and the dose of irradiation. The bands at 3080 cm−1 and 1744 cm−1, which originate from olefinic terminal bond (v = CH) and ester carbonyl group (vRO–C[dbnd]O), respectively, indicate the influence of oxidative stress on lipid degradation and peroxidation, respectively. The new bands at about 1690 cm−1 and 1516 cm−1 denote the presence of β-sheet conformation of the proteins due to the diseases, confirming the increasing amount of lipophilic environment and fibril formation. Comparison of the FT-IR spectra of calcified aortic valve and hip heterotopic ossification with that of normal bones showed that in the bone-like formation the peroxide anion free radicals play an important role in the disease. © 2017 Elsevier B.V.
ER -